Synthetic arabinomannan glycolipids impede mycobacterial growth, sliding motility and biofilm structure

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• 作者：Kirtimaan Syal ; Krishnagopal Maiti ; Kottari Naresh…
• 关键词：Biofilms ; Glycolipids ; Mycobacteria ; Oligosaccharides ; Sliding motility
• 刊名：Glycoconjugate Journal
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：33
• 期：5
• 页码：763-777
• 全文大小：1,348 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Pathology
  
• 出版者：Springer Netherlands
• ISSN：1573-4986
• 卷排序：33

文摘

Mycobacterium has evolved distinct cell wall and strategies such as biofilm formation, which helps it to survive in hostile conditions. We have reported previously that arabinofuranoside containing glycolipids exhibit inhibition activities against the above functions of the mycobacterial species M. smegmatis. In search for activities mediated by oligosaccharide glycolipids, we report herein the inhibitory activities of a linear and a branched pentasaccharides having arabinan and mannan moieties. In the presence of the pentasaccharide glycolipids, a significant reduction in mycobacterial growth is observed, concomitant with reductions in sliding motility and colonization through biofilm formation, at the optimal glycolipid concentrations of 50–100 μg mL−1. Especially the biofilm coat is ruptured by ~80–85 % in the presence of glycolipids. Pentasaccharides alone without the lipidic chain show only a weak effect. The glycolipids are non-toxic, as evaluated through their effect on RBCs. Analysis of the mycolic acid profile of glycolipid treated biofilm shows that α- and epoxy mycolic acids are downregulated significantly, in comparison to glycolipid untreated biofilms. Lipidomics profile analysis through mass spectrometry further reveals profound downregulation of phosphatidylinositol mannosides, acylatedphosphoglycerols and mycolic acid family, namely, keto-, alpha- and methoxymycolic acids.