HPLC of anthocyans with an amperometric detector: Evaluation of the antioxidant activity

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• 作者：L. A. Deineka ; S. L. Makarevich ; V. I. DeinekaR30
• 关键词：anthocyans ; Vitis vinifera ; antioxidant activity ; amperometric method ; Tsvet ; Yauza ; 01 ; AA
• 刊名：Journal of Analytical Chemistry
• 出版年：2015
• 出版时间：August 2015
• 年：2015
• 卷：70
• 期：8
• 页码：989-994
• 全文大小：235 KB
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• 作者单位：L. A. Deineka (1)
  S. L. Makarevich (1)
  V. I. Deineka (1)
  A. N. Chulkov (2)
  
  1. Department of Biology and Chemistry, Belgorod State National Research University, ul. Pobedy 85, Belgorod, 308015, Russia
  2. Federal Center for Evaluation of Safety and Quality of Grain and Products of Its Processing, Belgorod Branch, ul. Shchorsa 8, Belgorod, 308027, Russia
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Russian Library of Science
  
• 出版者：MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
• ISSN：1608-3199

文摘

Anthocyan complexes of a series of grape varieties with the composition traditional for Vitis vinifera, containing 3-glucosides of five anthocyanidins, delphinidin (Dp3G), cyanidin (Cy3G), petunidin (Pt3G), peonidin (Pn3G), and malvidin (Mv3G) as the major components have been studied by reversed-phase HPLC with diode-array, mass spectrometric, and amperometric detectors. A procedure has been developed for the determination of the antioxidant activity of individual anthocyans using an amperometric method; the peculiarities of using a Tsvet Yauza-01-AA system have been evaluated. It has been shown that the sensitivity of amperometric detection depends not only on the structure of anthocyans, but also on the anode voltage. A scheme of the calculation of the antioxidant activity of anthocyan complexes by the results of amperometric detection of a mixture of individual anthocyans has been proposed.