Cellulase On-Site Production from Sugar Cane Bagasse Using Penicillium echinulatum
详细信息 查看全文
- 作者：Beatriz Merchel Piovesan Pereira (1)
Thabata Maria Alvarez (1)
Priscila da Silva Delabona (1)
Aldo José Pinheiro Dillon (2)
Fabio Márcio Squina (1)
José Geraldo da Cruz Pradella (1) - 关键词：Penicillium echinulatum ; Cellulase ; Enzyme ; Submerged fermentation ; Second generation bioethanol
- 刊名：BioEnergy Research
- 出版年：2013
- 出版时间：September 2013
- 年：2013
- 卷：6
- 期：3
- 页码：1052-1062
- 全文大小：599KB
- 参考文献：1. Macedo IC (organizer) (2007) Sugarcane’s energy—twelve studies on Brazilian sugarcane agribusiness and its sustainability. 2nd edition, Editora Berlendis, S?o Paulo, SP, Brazil (http://sugarcane.org/resource-library/books/Sugar%20Canes%20Energy%20-%20Full%20book.pdf)
2. Zhang YHP, Himmel ME, Mielenz JR (2006) Outlook for cellulase improvement: screening and selection strategies. Biotechnol Adv 24:452-81 CrossRef
3. Tolan JS (2002) Iogen’s process for producing ethanol from cellulosic biomass. Clean Techn Environ Policy 3:339-45 CrossRef
4. Sorensen A, Teller PJ, Lubeck PS, Ahring BK (2011) Onsite enzyme production during bioethanol production from biomass: screening for suitable fungal strains. Appl Biochem Biotechnol 164:1058-070 CrossRef
5. Martins LF, Kolling D et al (2008) Comparison of / Penicillium echinulatum and / Trichoderma reesei cellulases in relation to their activity against various cellulosic substrates. Bioresour Technol 19:1417-424 CrossRef
6. Camassola M, Bittencourt LR et al (2004) Characterization of the cellulase complex of / Penicillium echinulatum. Biocatal Biotransform 22:391-96 CrossRef
7. Sehnem NT, Bittencourt LR et al (2006) Cellulase production by / Penicillium echinulatum on lactose. Appl Microbiol Biotechnol 72:163-67 CrossRef
8. Camassola M, Dilon AJP (2007) Effect of methylxanthines on production of cellulases by / Penicillium echinulatum. J Appl Microbiol 102:478-85 CrossRef
9. Dillon AJP, Zorgi C (2006) Use of 2-deoxyglucose in liquid media for the selection of mutant strains of / Penicillium echinulatum producing increased cellulase and b-glucosidase activities. Appl Microbiol Biotechnol 70:740-46 CrossRef
10. Mandels M, Reese ET (1957) Induction of cellulase in / Trichoderma viride as influenced by carbon sources and metals. J Bacteriol 73:269-78
11. Ghose TK (1987) Measurement of cellulose activities. Pure Appl Chem 59:257-68 CrossRef
12. Bailey MJ, Poutanen K (1989) Production of xylanolytic enzymes by strains of / Aspergillus. Appl Microbiol Biotechnol 30:5-0 CrossRef
13. Zhang Y-HP, Hong J, Ye X (2009) Cellulase assays. Methods Mol Biol 581:213-31 CrossRef
14. Ahamed A, Vermette P (2008) Culture-based strategies to enhance cellulase enzyme production from / Trichoderma reesei RUT-C30 in bioreactor culture conditions. Biochem Eng J 40:399-07 CrossRef
15. Gouveia ER, Nascimento RT, Souto-Maior AM, Rocha GJM (2009) Validation of the methodology for the chemical characterization of the sugarcane bagasse. Quím Nova 32:1500-503 CrossRef
16. Bigelow M, Wyman CE (2002) Cellulase production on bagasse pretreated with hot water. Appl Biochem Biotechnol 98:921-34 CrossRef
17. Palmqvist E, Hahn-H?gerdal B (2000) Fermentation of lignocellulosic hydrolysates. II: inhibitors and mechanisms of inhibition. Bioresour Technol 74:25-3 CrossRef
18. Grethlein HE (1985) The effect of pore size distribution on the rate of enzymatic hydrolysis of cellulosic substrates. Biotechnology 3:155-60 CrossRef
19. Ilmén M, Thrane C, Penttil? ME (1996) The glucose repressor gene cre1 of / Trichoderma: isolation and expression of a full-length and a truncated mutant form. Mol Gen Genet 251:451-60
20. Lejeune R, Baron GV (1995) Effect of agitation on growth and enzyme production of / Trichoderma reesei in batch fermentation. Appl Microbiol Biotechnol 43:249-58 CrossRef
21. Camassola M, Dillon AJP (2007) Production of cellulases and hemicellulases by / Penicillium echinulatum grown on pretreated sugarcane bagasse and wheat bran in solid state fermentation. J Appl Microbiol 103:2196-204 CrossRef
22. Kovács K, Szacks G, Zacchi G (2009) Comparative enzymatic hydrolysis of pretreated spruce by supernatants, whole fermentation broth and washed mycelia of / Trichoderma reesei and / Trichoderma atrovide. Bioresour Technol 100:1350-357 CrossRef
23. Ahmed S, Bashir A, Saleem H, Saadia M, Jamil A (2009) Production and purification of cellulose degrading enzymes from a filamentous fungus / Trichoderma harzianum. Pak J Bot 41:1411-419
24. Castro AM, Ferreira MC, Cruz JC, Nascimento KC, Rodrigues P, Carvalho DF, Leite SGF, Pereira N Jr (2010) High-yield endoglucanase production by / Trichoderma harzianum IOC-3844 cultivated in pretreated sugarcane mill byproduct. Enzym Res. doi:10.4061/2010/854526
25. Delabona PS, Farinas CS, Silva MR, Azzoni SF, Pradella JGC (2012) Use of a new / Trichoderma harzianum strain isolated from the Amazon rainforest with pretreated sugarcane bagasse for on-site cellulase production. Bioresour Technol 107:517-21 CrossRef
26. Cosgrove DJ (2005) Growth of the plant cell wall. Nat Rev Mol Cell Biol 6:850-61 CrossRef
27. Walton JD (1994) Deconstructing the cell wall. Plant Physiol 104:1113-118
28. Berlin A, Gilkes N, Kilburn D, Bura R, Markov A, Skomarovsky A, Okunev O, Gusakov A, Greg D, Sinitsyn A, Saddler J (2005) Evaluation of novel fungal cellulase preparations for ability to hydrolyze softwood substrates—evidence for the role of accessory enzymes. Enzyme Microb Technol 37:175-84 CrossRef
29. Kumar R, Wyman CE (2009) Effect of enzyme supplementation at moderate cellulase loadings on initial glucose and xylose release from corn stover solid pretreated by leading technologies. Biotechnol Bioeng 102:457-67 CrossRef - 作者单位：Beatriz Merchel Piovesan Pereira (1)
Thabata Maria Alvarez (1)
Priscila da Silva Delabona (1)
Aldo José Pinheiro Dillon (2)
Fabio Márcio Squina (1)
José Geraldo da Cruz Pradella (1)
1. Brazilian Bioethanol Science and Technology Laboratory, Rua Giuseppe Maximo Scolfaro 10000, Pólo II de Alta Tecnologia, CP 6192, Campinas, S?o Paulo, 13083-970, Brazil
2. Biotechnology Institute, University of Caxias do Sul, Rua Francisco Getulio Vargas 1130, Caxias do Sul, Rio Grande do Sul, 95010-550, Brazil
文摘
Penicillium echinulatum was evaluated as a cellulolytic enzyme producer in shaking flasks and bioreactor submerged culture using sugarcane bagasse as carbon source. Sodium hydroxide delignified steam-exploded pretreated bagasse (SDB) and hydrothermal pretreated bagasse had a maximum filter paper activity (FPase) of 2.4 and 2.6 FPU/mL, respectively. Delignified acid pretreated bagasse and Celufloc 200TM (CE) carbon sources displayed maximum FPase of 1.3 and 1.6 FPU/mL while in natura bagasse (INB) provided the lowest enzyme activity, ca. 0.4 FPU/mL. Measurement of surface specific area of lignocellulosic material and scanning electron microscopic images showed a possible correlation between fungal mycelia accessibility to lignocellulosic particles and obtained cellulolytic enzyme activity of fermentation broth. Fed-batch experiments performed in a controlled bioreactor attained the highest value of FPase of 3.7 FPU/mL, enzyme productivity of 25.7 FPU/L?h, and enzyme yield from cellulose equal to 134 FPU/g with SDB. Enzyme hydrolysis of steam-pretreated bagasse accomplished with the obtained supernatant of fermentation broth (10 FPU/g of biomass and 5?%?w/v) performed better than commercial cellulose complex. The results showed that P. echinulatum has potential to be used as an on-site enzyme platform aiming second bioethanol production from sugarcane lignocellulosic residue.