Permanganate-assisted removal of PCR inhibitors during the DNA Chelex extraction from stained denim samples

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• 作者：Sorina Pîrlea ; Mihaela Puiu ; Adina Răducan…
• 关键词：Phthalocyanine ; Chelex ; Polymerase chain reaction ; DNA amplification
• 刊名：International Journal of Legal Medicine
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：131
• 期：2
• 页码：323-331
• 全文大小：
• 刊物类别：Medicine
• 刊物主题：Forensic Medicine; Medical Law; Medicine/Public Health, general;
• 出版者：Springer Berlin Heidelberg
• ISSN：1437-1596
• 卷排序：131

文摘

In this study, it was demonstrated that the DNA Chelex extraction combined with the permanganate assisted-oxidation is highly efficient in removing the PCR inhibitors often found in clothing materials, such as phthalocyanine. The extraction assays were conducted in saliva, blood and epithelial cells samples mixed with three oxidation-resistant dye copper(II) α-phthalocyanine, copper(II) β-phthalocyanine and tetrasulfonated copper(II) β-phthalocyanine. After DNA amplification, all samples were able to provide full DNA profiles. The permanganate/Chelex system was tested further on denim-stained samples and displayed the same ability to remove the PCR inhibitors from the commercial textile materials.