Functional Divergence and Evolutionary Dynamics of the Putative AAAP Gene Family in Brassica rapa

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• 作者：Li-Zong Hu (1) (2)
  Wei-Bo Yin (1)
  Yu-Hong Chen (1)
  Zan-Min Hu (1)
  
• 关键词：Brassica rapa ; AAAP proteins ; Selection ; Functional divergence
• 刊名：Plant Molecular Biology Reporter
• 出版年：2014
• 出版时间：April 2014
• 年：2014
• 卷：32
• 期：2
• 页码：517-530
• 全文大小：1,358 KB
• 作者单位：Li-Zong Hu (1) (2)
  Wei-Bo Yin (1)
  Yu-Hong Chen (1)
  Zan-Min Hu (1)
  
  1. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Chaoyang District, Beijing, 100101, China
  2. University of Chinese Academy of Sciences, Beijing, 100049, China
  
• ISSN：1572-9818

文摘

The amino acid/auxin permease (AAAP) protein family is ubiquitously present in almost all eukaryotic species and functions in various aspects of growth and development. To investigate the evolution of AAAP proteins, here 83 AAAP genes in Brassica rapa were identified, and their sequence features, and evolutionary relationships were analyzed using in silico methods. According to the phylogenetic analysis, the AAAP genes of B. rapa are divided into six clades, and these clades share relatively similar sequence features, including gene structures, conserved motifs, and domain organizations. Synteny mapping strongly suggested that segmental duplications could be responsible for the expansion of this family. Adaptive evolution analysis demonstrated that most of AAAP proteins were subject to purifying selection. However, the site Tyr257 on eight AAAP proteins from clade 2b underwent significant positive selection. Functional divergent analysis showed that type I functional divergence coefficients (θ I ) were significantly greater than zero in six pair-wise comparisons. However, functional divergence sites (Q k -gt;-.95) found only in the AAAP I/II and AAAP I/III comparisons were localized mainly to the trans-membrane (TM) regions, suggesting highly divergent TM structures between these groups might be associated with group-specific functions. Our results could provide a valuable clue for further investigations of the evolutionary history and biological functions of the AAAP genes in B. rapa.