Validation Assay of p3_VvAGL11 Marker in a Wide Range of Genetic Background for Early Selection of Stenospermocarpy in Vitis vinifera L.

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• 作者：Carlo Bergamini (1)
  Maria Francesca Cardone (1)
  Angelo Anaclerio (1) (2)
  Rocco Perniola (1)
  Arianna Pichierri (1)
  Rosalinda Genghi (1)
  Vittorio Alba (1)
  Lucia Rosaria Forleo (1)
  Angelo Raffaele Caputo (1)
  Cinzia Montemurro (2)
  Antonio Blanco (2)
  Donato Antonacci (1)
  
• 关键词：Marker ; assisted selection ; Stenospermocarpy ; Seedless ; Grapevine breeding
• 刊名：Molecular Biotechnology
• 出版年：2013
• 出版时间：July 2013
• 年：2013
• 卷：54
• 期：3
• 页码：1021-1030
• 全文大小：476KB
• 参考文献：1. Mejía, N., Soto, B., Guerrero, M., Casanueva, X., Houel, C., Miccono, M. E. L., et al. (2011). Molecular, genetic and transcriptional evidence for a role of VvAGL11 in stenospermocarpic seedlessness in grapevine. / BMC Plant Biology, / 11, 57. CrossRef
  2. Costantini, L., Battilana, J., Lamaj, F., Fanizza, G., & Grando, M. S. (2008). Berry and phenology-related traits in grapevine ( / Vitis vinifera L.): From quantitative trait loci to underlying genes. / BMC Plant Biology, / 8, 38. CrossRef
  3. Moroldo, M., Paillard, S., Marconi, R., Fabrice, L., Canaguier, A., Cruaud, C., et al. (2008). A physical map of the heterozygous grapevine ‘Cabernet Sauvignon-allows mapping candidate genes for disease resistance. / BMC Plant Biology, / 8, 66. CrossRef
  4. Carmona, M. J., Cha?b, J., Martínez-Zapater, J. M., & Thomas, M. R. (2008). A molecular genetic perspective of reproductive development in grapevine. / Journal of Experimental Botany, / 59, 2579-596. CrossRef
  5. Emanuelli, F., Battilana, J., Costantini, L., Le Cunff, L., Boursiquot, J. M., This, P., et al. (2010). A candidate gene association study on muscat flavor in grapevine ( / Vitis vinifera L.). / BMC Plant Biology, / 10, 241. CrossRef
  6. Battilana, J., Emanuelli, F., Gambino, G., Gribaudo, I., Gasperi, F., Boss, P. K., et al. (2011). Functional effect of grapevine 1-deoxy-d -xylulose 5-phosphate synthase substitution K284?N on Muscat flavour formation. / Journal of Experimental Botany, / 62, 5497-508. CrossRef
  7. Akkurt, M., Cak?r, A., Shidfar, M., Celikkol, B. P., & S?ylemezo?lu, G. (2012). Using SCC8, SCF27 and VMC7f2 markers in grapevine breeding for seedlessness via marker assisted selection. / Genetics and Molecular Research, / 11, 2288-294. CrossRef
  8. Karaagac, E., Vargas, A., Andrés, M., Carre?o, I., Ibá?ez, J., Carre?o, J., et al. (2012). Marker assisted selection for seedlessness in table grape breeding. / Tree Genetics and Genomes, / 8, 1003-015. CrossRef
  9. Fatahi, R., Zamani, Z., Ebadi, A., & Mehlenbacher, S. A. (2004). The inheritance of seedless Scc8-scar and Ssrs loci alleles in progeny of ‘Muscat hamburg’?×?’bidane quermez-grapes. / Ishs Acta Horticulturae, / 652, 329-35.
  10. Adam-blondon, A., Lahogue-esnault, F., Bouquet, A., & Boursiquot, J. (2001). Usefulness of two SCAR markers for marker-assisted selection of seedless grapevine cultivars. / Vitis, / 40, 147-55.
  11. Cabezas, J. A., Cervera, M. T., Ruiz-García, L., Carre?o, J., & Martínez-Zapater, J. M. (2006). A genetic analysis of seed and berry weight in grapevine. / Genome, / 49, 1572-585. CrossRef
  12. Collard, B. C., & Mackill, D. J. (2008). Marker-assisted selection: an approach for precision plant breeding in the twenty-first century. / Philosophical Transactions of the Royal Society of London. Series B, Biological sciences, / 363, 557-72. CrossRef
  13. Migliaro, D., Santellani, F., Da Rold, G., Velasco R., Vezzulli, S., & Crespan M. (2012). Experimental assessment of molecular markers efficiency (VvAGL11) in MAS for table grape breeding. XXXV Word Congress of the Vine and Wine (June 2012, Izmir, Turkey).
  14. Fanizza, G., Lamaj, F., Costantini, L., Chaabane, R., & Grando, M. S. (2005). QTL analysis for fruit yield components in table grapes ( / Vitis vinifera). / Theoretical and Applied Genetics, / 111, 658-64. CrossRef
  15. Le Cunff, L., Fournier-Level, A., Laucou, V., Vezzulli, S., Lacombe, T., Adam-Blondon, A. F., et al. (2008). Construction of nested genetic core collections to optimize the exploitation of natural diversity in / Vitis vinifera L. subsp. sativa. / BMC Plant Biology, / 8, 31. CrossRef
  16. Laucou, V., Lacombe, T., Dechesne, F., Siret, R., Bruno, J. P., Dessup, M., et al. (2011). High throughput analysis of grape genetic diversity as a tool for germplasm collection management. / Theoretical and Applied Genetics, / 122, 1233-245. CrossRef
  17. Thomas, M. R., & Scott, N. S. (1993). Microsatellite repeats in grapevine reveal DNA polymorphisms when analysed as sequence-tagged sites (STSs). / Theoretical and Applied Genetics, / 86, 985-90.
  18. Bowers, J. E., Dangl, G. S., Vignani, R., & Meredith, C. P. (1996). Isolation and characterization of new polymorphic simple sequence repeat loci in grape ( / Vitis vinifera L.). / Genome, / 39, 628-33. CrossRef
  19. Bowers, J. E., Dangl, G. S., & Meredith, C. P. (1999). Development and characterization of additional microsatellite DNA markers for grape. / American Journal of Enology and Viticulture, / 50, 243-46.
  20. Sefc, K. M., Regner, F., Turetschek, E., Gl?ssl, J., & Steinkellner, H. (1999). Identification of microsatellite sequences in / Vitis riparia and their applicability for genotyping of different Vitis species. / Genome, / 42, 367-73.
  21. Pellerone, F., Edwards, K., & Thomas, M. (2001). Grapevine microsatelliterepeats: isolation, characterisation and use for genotyping of grape germplasm from Southern Italy. / Vitis, / 40, 179-86.
  22. Wagner, H. W., & Sefc, K. M. (1999). / IDENTITY 1.0. Centre for applied genetics. Vienna: University of Agricultural Sciences.
  23. Falush, D., Stephens, M., & Pritchard, J. K. (2003). Inference of population structure using multilocus genotype data: linked loci and correlated allele frequencies. / Genetics, / 164, 1567-587.
  24. Pritchard, J. K., Stephens, M., & Donnelly, P. (2000). Inference of population structure using multilocus genotype data. / Genetics, / 155, 945-59.
  25. Landridge, P. & Chalmers, K. J. (2005). The principle: Identification and application of molecular markers. / Biotechnology in agriculture and forestry (pp. 3-2). Heidelberg: Springer-Verlag.
  
• 作者单位：Carlo Bergamini (1)
  Maria Francesca Cardone (1)
  Angelo Anaclerio (1) (2)
  Rocco Perniola (1)
  Arianna Pichierri (1)
  Rosalinda Genghi (1)
  Vittorio Alba (1)
  Lucia Rosaria Forleo (1)
  Angelo Raffaele Caputo (1)
  Cinzia Montemurro (2)
  Antonio Blanco (2)
  Donato Antonacci (1)
  
  1. Consiglio per la Ricerca e la sperimentazione in Agricoltura - CRA, Unità di ricerca per l’uva da tavola e la vitivinicoltura in ambiente mediterraneo, Research unit for viticulture and enology in southern Italy, Via Casamassima, 148, 70010, Turi, BA, Italy
  2. Dipartimento di Scienze del Suolo, della Piante e degli Alimenti, Sez. Genetica e Miglioramento Genetico, Università di Bari “Aldo Moro- Via Amendola 165/A, 70126, Bari, Italy
  

文摘

DNA markers technology, derived from research in molecular biology and genomics, offers great promise for plant breeding, allowing the “molecular breeding-via marker-assisted selection. Grapevine genomic resources allowed, in recent years, the characterization at molecular level of genes involved in interesting phenotypes such as stenospermocarpic seedlessness, a trait really appreciated by consumers. Recent studies in table grapes revealed that the VvAGL11 gene, member of the D-lineage MADS-box family, controls the ovule identity, and thus potentially playing an important role in stenospermocarpy. Intragenic markers of VvAGL11 have been found and tested for breeding purposes. In the present paper, we describe an in deep assay on a total of 475 genotypes derived by our own grape germplasm and seeded?×?seedless crosses F1 offspring, to evaluate and verify the “diagnostic-power of VvAGL11 in marker-assisted selection. We found only 8/475 that were seeded and carried the seedless-associated allele in the STS p3_VvAGL11. However, and most importantly, there were no seedless varieties without such allele. We validated the marker as a 100?% effective tool for early negative selection of stenospermocarpy in Vitis vinifera L. crosses.