Morphological Variability in Leaves and Molecular Characterization of Novel Table Grape Candidate Cultivars (Vitis vinifera L.)

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• 作者：Vittorio Alba (1)
  Carlo Bergamini (1)
  Maria Francesca Cardone (1)
  Marica Gasparro (1)
  Rocco Perniola (1)
  Rosalinda Genghi (1)
  Donato Antonacci (1)
  
• 关键词：Table grape ; Ampelography ; Ampelometry ; Microsatellites
• 刊名：Molecular Biotechnology
• 出版年：2014
• 出版时间：June 2014
• 年：2014
• 卷：56
• 期：6
• 页码：557-570
• 全文大小：
• 参考文献：1. Myles, S., Boyko, A. R., Owens, C. L., Brown, P. J., Grassi, F., Aradhya, M. K., et al. (2010). Genetic structure and domestication history of the grape. / Proceedings of the National Academy of Sciences of the United States of America, / 108(9), 3457-458.
  2. Pelengi?, R., & Rusjan, D. (2010). Efficacy of ampelographic and phyllometric tools for the validation of grapevine / Vitis vinifera L. biodiversity in Slovenia. / Journal of Food, Agriculture & Environment, / 4, 563-68.
  3. Santiago, J. L., Gonzalez, I., Gago, P., Alonso-Villaverde, V., Boso, S., & Martinez, M. C. (2008). Identification of and relationships among a number of / teinturier grapevines that expanded across Europe in the early tweentith century. / Australian Journal of Grape and Wine Research, / 14, 223-29.
  4. Sefc, K. M., Lefort, M. S., Grando, K., Scott, H., Steinkellner, & Thomas, M. R. (2001). Microsatellite markers for grapevine: A state of the art. In K. A. Roubelakis-Angelakis (Ed.), / Molecular biology and biotechnology of grapevine (pp. 407-38). Amsterdam: Kluwer.
  5. Toma?i?, I., & Koro?ec-Koruza, Z. (2003). Validity of phyllometric parameters used to differentiate local / Vitis vinifera L. cultivars. / Genetic Resources and Crop Evolution, / 50, 773-78. CrossRef
  6. Keller, M. (2010). / The science of grapevines: Anatomy and physiology. Rio de Janeiro: Elsevier Science.
  7. Ort?z, J. M., Mart?n, J. P., Borrego, J., Chavez, J., Rodr?guez, I., Munoz, G., et al. (2004). Molecular and morphological characterization of a / Vitis gene bank for the establishment of a base collection. / Genetic Resources and Crop Evolution, / 51, 403-09. CrossRef
  8. Santiago, J. L., Boso, S., Martin, J. P., Ortiz, J. M., & Martinez, M. C. (2005). Characterization and identification of grapevine cultivars ( / Vitis vinifera L.) from Northwestern Spain using microsatellite marlers and ampelometric methods. / Vitis, / 44(2), 67-2.
  9. OIV (2009) 2nd edition of the OIV descriptor list for grape varieties and / Vitis species. Organisation Internationale de la Vigne et du Vin, Paris, France—International Plant Genetic Resources Institute, Rome, Italy (www.oiv.org).
  10. Galet, P. (1998). / Grape varieties and rootstock varieties. Collection avenis oenologie. Chaintré: Oeno Plurimedia.
  11. Frankel, O. H., & Brown, A. H. D. (2001). Plant genetic resources today: a critical appraisal. In J. H. W. Holden & J. T. Williams (Eds.), / In crop genetic resources: Conservation and evaluation (pp. 249-57). London: Georges Allen & Unwin Ltd.
  12. Polignano, G. B., Uggenti, P., Alba, V., Bisignano, V., & Della Gatta, C. (2005). Morphoagronomic diversity in grasspea ( / Lathyrus sativus L.). / Plant Genetic Resources, / 3(1), 29-4. CrossRef
  13. Le Cunff, L., Fournier-Level, A., Laucou, V., Vezzulli, S., Lacombe, T., Adam-Blondon, A. F., et al. (2008). Construction of nested genetic core collections to optimize the exploitation of natural diversity in / Vitis vinifera L. subsp. / Sativa. / BMC Plant Biology, / 8, 31. doi:10.1186/1471-2229-8-31 . CrossRef
  14. Rohlf, F. J. (2001). NTSYS-PC: Numerical taxonomy and multivariate analysis system, version 2.02i. In A. C. Godwin (Ed.), / Department of ecology and evolution (pp. 407-38). New York: State University of New York.
  15. Superampelos 2.0. Programma di ampelometria fogliare. Comunità Monastica S.S. Pietro e Paolo. Loc. Giardino della Risurrezione, 28887 Germagno (VB), Italy, http://www.pomologia.it/SuperAmpelo/Release.aspx.
  16. Thomas, M. R., & Scott, N. S. (1993). Microsatellite repeats in grapevine reveal DNA polymorphisms when analysed as sequence-tagged sites (STSs). / Theoretical and Applied Genetics, / 86, 985-90. CrossRef
  17. Bowers, J. E., Dangl, G. S., Vignani, R., & Meredith, C. P. (1996). Isolation and characterization of new polymorphic simple sequence repeat loci in grape ( / Vitis vinifera L.). / Genome, / 39, 628-33. CrossRef
  18. Bowers, J. E., Dangl, G. S., & Meredith, C. P. (1999). Development and characterization of additional microsatellite DNA markers for grape. / American Journal of Enology and Viticulture, / 50(3), 243-46.
  19. Sefc, K. M., Regner, E., Turetschek, J., Gl?ssl, H., & Steinkellner, (1999). Identification of microsatellite sequences in / Vitis riparia and their applicability for genotyping of different / Vitis species. / Genome, / 42, 367-73. CrossRef
  20. Yeh, F. C., Yang, R. C., & Boyle, T. (1999). POPGENE version 1.31: Microsoft Windows—based freeware for population genetic analysis. In M. J. Wingfield (Ed.), / Department of renewable resources. Edmonton: University of Alberta.
  21. Dice, L. R. (1945). Measures of the amount of ecologic association between species. / Ecology, / 26, 297-02. CrossRef
  22. Alleweldt, G., & Dettweiler, E. (1989). A model to differentiate grapevine cultivars with the aid of morphological characteristics. / Rivista di Viticoltura é Enologia, / 42, 59-3.
  23. Davies, T. J., & Savolainen, V. (2006). Neutral theory, phylogenies, and the relationship between phenotypic change and evolutionary rates. / Evolution, / 60(3), 476-83. CrossRef
  24. Costacurta, A., Calò, A., Carraro, R., Giust, M., Antoniazzi, M., & Lazzaro, G. (1996). Metodologie computerizzate per la caratterizzazione dei vitigni (1° contributo). / Rivista di Viticoltura é Enologia, / 49, 27-4.
  25. Galet, P. (1988). / Cépages et vignobles de frances (pp. 9-7). Montpellier: Dèhan.
  26. Bowers, J., Boursiquot, J. M., This, P., Chu, K., Johansson, H., & Meredith, C. (1999). Historical genetics: The Parentage of chardonnay, gamay, and other wine grapes of Northeastern France. / Science, / 285(5433), 1562-565. CrossRef
  27. Meredith, C. P., Bowers, J. E., Riaz, S., Handley, V., Bandman, E. B., & Dangl, G. S. (1999). The identity and parentage of the variety known in California as Petite Sirah. / American Journal of Enology and Viticulture, / 50, 236-42.
  28. This, P., Jung, A., Boccacci, P., Borrego, J., Botta, R., Costantini, L., et al. (2004). Development of a standard set of microsatellite reference alleles for identification of grape cultivars. / Theoretical and Applied Genetics, / 109, 1448-458. CrossRef
  29. Fernandez-Gonzalez, M., Mena, A., Izquierdo, P., & Martinez, J. (2007). Genetic characterization of grapevine ( / Vitis vinifera L.) cultivars from Castilla La Mancha (Spain) using microsatellite markers. / Vitis, / 46(3), 126-30.
  30. Jak?e, J. N., ?tajner, L., Tomi?, & Javornik, B. (2013). Application of microsatellite markers in grapevine and olives. In Barbara Sladonja (Ed.), / The Mediterranean genetic code—grapevine and olive. Zagreb: InTech. doi:10.5772/53411 . ISBN 978-953-51-1067-5.
  31. Stajner, N., Rusjan, D., Korosec-Koruza, Z., & Javornik, B. (2011). Genetic characterization of old slovenian grapevine varieties of / Vitis vinifera L. by Microsatellite Genotyping. / American Journal of Enology and Viticulture, / 62(2), 250-55. CrossRef
  32. Santana, J. C., Heuertz, M., Arranz, C., Rubio, J. A., Martinez-Zapater, J. M., & Hidalgo, E. (2010). Genetic structure, origins, and relationships of grapevine cultivars from the Castilian Plateau of Spain. / American Journal of Enology and Viticulture, / 61(2), 214-24.
  33. Laucou, V., Lacombe, T., Dechesne, F., Siret, R., Bruno, J. P., Dessup, M., et al. (2011). High throughput analysis of grape genetic diversity as a tool for germplasm collection management. / Theoretical and Applied Genetics, / 122(6), 1233-245. CrossRef
  
• 作者单位：Vittorio Alba (1)
  Carlo Bergamini (1)
  Maria Francesca Cardone (1)
  Marica Gasparro (1)
  Rocco Perniola (1)
  Rosalinda Genghi (1)
  Donato Antonacci (1)
  
  1. Consiglio per la Ricerca e la sperimentazione in Agricoltura - CRA, Unità di ricerca per l’uva da tavola e la vitivinicoltura in ambiente mediterraneo, Via Casamassima 148, 70010, Turi, BA, Italy
  
• ISSN：1559-0305

文摘

The present work report the characterization of twenty-one table grapes candidate cultivars plus five registered ones included as reference, by means of 47 ampelographic traits, 23 ampelometric measurements and six microsatellite loci. The final goal of the research was to analyse the possibility of reducing the number of morphological and molecular tools required for a precise and effective description of a grape genotype or cultivar. This would be of great help for future biodiversity description on a larger sample of more than 300 table grapes accessions today grown at the ‘Consiglio per la Ricerca e la sperimentazione in Agricoltura (C.R.A.)—Unità di ricerca per l’uva da tavola e la vitivinicoltura in ambiente mediterraneo (Bari—Italy)- OIV ampelographic traits showed a clear distinction among all twenty-six genotypes analysed, suggesting the relevant morphological variability investigated. Principal component analysis based on ampelometric traits revealed main veins ON3, ON4 and O3N4; ratios between main veins; angles between main veins and of petiolar sinus, to be the most effective records in differentiating cultivars, for a total variation of 69.9?% described by the first three components. Molecular analysis based on six microsatellite loci was performed on all genotypes, providing a detailed molecular profile and a dendrogram of genetic similarity, in which all genotypes were clearly distinguishable. Finally, with the goal of using the minimum possible number of markers to differentiate genotypes, microsatellites VVMD5 and VVMD27 were selected to be sufficient to distinguish among all the candidate cultivars included in the analysis, representing a possible ‘step by step-approach when a molecular characterization has to be undertaken on a large number of genotypes, by first testing few markers and increasing their number only if necessary.