Enhanced Chemiluminescence Determination of Hydrogen Peroxide in Milk Sample Using Metal–Organic Framework Fe–MIL–88NH₂ as Peroxidase Mimetic

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• 作者：Yinhuan Li ; Xiaoying You ; Xiangyang Shi
• 关键词：Chemiluminescence ; Hydrogen peroxide ; Metal–organic framework ; Milk
• 刊名：Food Analytical Methods
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：10
• 期：3
• 页码：626-633
• 全文大小：
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Food Science; Chemistry/Food Science, general; Microbiology; Analytical Chemistry;
• 出版者：Springer US
• ISSN：1936-976X
• 卷排序：10

文摘

The presence of hydrogen peroxide (H₂O₂) in milk is a major concern because it constitutes a public health hazard. Here, a sensitive flow injection chemiluminescence method was established for the detection of H₂O₂. As a peroxidase mimetic, metal–organic framework Fe–MIL–88NH₂ was found to significantly enhance the chemiluminescence of luminol–H₂O₂ reaction. The enhancement mechanism could be attributed to peroxidase-like activity of Fe–MIL–88NH₂, which effectively catalyzed the decomposition of H₂O₂ into hydroxyl radical. The experimental conditions for the chemiluminescence reaction were thoroughly investigated. The chemiluminescence intensity was proportional to the concentration of H₂O₂ in the range of 0.1–10.0 μmol/L. The detection limit was 0.025 μmol/L H₂O₂, and the relative standard deviation was 2.6 % for 11 replicated measurements of 1.0 μmol/L H₂O₂ solution. The practicability of this method was demonstrated by determining H₂O₂ in milk samples.