Manganese Peroxidases from Ganoderma applanatum Degrade β-Carotene Under Alkaline Conditions

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• 作者：Isabel Lanfermann ; Diana Linke ; Manfred Nimtz…
• 关键词：Biocatalysis ; Enzyme activity ; Carotene degradation ; Manganese peroxidase ; Purification ; Filamentous fungi ; Ganoderma applanatum ; Washing ; Alkaline activity
• 刊名：Applied Biochemistry and Biotechnology
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：175
• 期：8
• 页码：3800-3812
• 全文大小：683 KB
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• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Biochemistry
  
• 出版者：Humana Press Inc.
• ISSN：1559-0291

文摘

A β-carotene-degrading enzyme activity was observed in liquid cultures of the basidiomycete Ganoderma applanatum. Supplementing the cultures with β-carotene induced the bleaching activity. Purification via hydrophobic interaction, ion exchange and size exclusion chromatography followed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) resulted in a single protein band. LC-ion-trap-MS analyses and gene amplification identified two manganese peroxidase isoenzymes with 97.8?% identity on the amino acid level. These showed an estimated molecular mass of 48?kDa and an isoelectric point of 2.6. Properties not yet described for other manganese peroxidases were hydrogen-peroxide-independent catalysis and two maxima of the bleaching activity, a distinct one at pH 5 and a lower one at pH 8. During simulated washing studies, the applicability of the isoenzymes for the brightening of carotenoids under alkaline conditions was proven. The new enzymes may replace common bleaching agents to produce environmentally more compatible detergent formulations.