Biodegradation of Natural Rubber by Laccase and Manganese Peroxidase Enzyme of Bacillus subtilis

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• 作者：G. Nayanashree ; B. Thippeswamy
• 关键词：Bacillus subtilis ; Hevea brasiliensis ; Vulcanization ; Polyisoprene ; Laccase ; Manganese peroxidase
• 刊名：Environmental Processes
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：2
• 期：4
• 页码：761-772
• 全文大小：906 KB
• 参考文献：Aneja KR (2004) Experiments on Microbiology, Plant Pathology and Biotechnology. 4th ed. New Age International Pvt. Ltd. New Delhi. 1-07
  Anonymous (2011) Malaysian rubber board, 47000. Sungei Buloh, Selangor
  Anonymous (2013) Complete information on area and production of rubber in India, Essay on Science Laboratory
  Berekaa MM, Lions A, Reichelt R, Keller U, Steinbuchel A (2000) Effect of pretreatment of rubber material on its biodegradability by various rubber degrading bacteria. FEMS Microbiol Lett 184:199-06CrossRef
  Borel M, Kergomard A, Renard F (1981) Degradation of natural rubber by bacteria imperfecti. Agric Biol Chem 46:877-81CrossRef
  Breberg K, Andersons EB, Landfors E, Holst O (2002) Microbial detoxification of waste rubber material by wood rotting fungi. Bioresour Technol 83:221-24CrossRef
  Lions A, Rudolf Reichelt R, Keller U, Steinbuchel A (2000) A Gram negative bacterium identified as Pseudomonas aeruginosa, AL98, is a potent degrader of natural rubber and synthetic cis-1,4-polyisoprene. FEMS Microbiol Lett 182:155-61CrossRef
  Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Gen Microbiol 31:3017-027
  Pan L, Gung GJ, Yin B, Cheng PS (2009) Contribution to deterioration of polymeric materials by slow growing bacteria nocardia corynebacterioides. Int Biodeterior Biodegrad 63:24-9CrossRef
  Papinutti L, Martinez JM (2006) Production and characterization of laccase and manganese peroxidase from the ligninolytic fungus fomes sclerodermeus. J Technol Biotechnol 81:1064-070CrossRef
  Peter HA, James T, Stanley T (1994) Bergey’s Manual of Determinative Bacteriology. 9th edition: 1-54
  Rose K, Steinbüchel A (2005) Biodegradation of natural rubber and related compounds: recent insights into a hardly understood catabolic capability of microorganisms. Appl Environ Microbiol 71:2803-812CrossRef
  Roy VR, Das M, Banerjee R, Bhowmick A (2005) Comparative studies on rubber biodegradation through solid state and submerged fermentation. Process Biochem 42:181-86
  Shah AA, Hasan F, Hameed A, Ahmed S (2008) Biological degradation of plastics: a comprehensive review. Biotechnol Adv 26(246):265
  Shraddha SR, Sehgal S, Kamtania M, Kumar A (2011) Laccase microbial source production, purification, potential biotechnological application. Enzym Res 2011:1-1CrossRef
  Tsuchii A, Tokiwa Y (1999) Colonization and disintegration of tire rubber by a colonial mutant of nocardia. J Biosci Bioeng 87:542-44CrossRef
  Tsuchii A, Tokiwa Y (2001) Microbial degradation of tire rubber particles. Biotechnol Lett 23:963-69CrossRef
  Tsuchii A, Takeda K, Suzuki T, Tokiwa Y (1996) Colonization and degradation of rubber pieces by nocardia species. Biodegrad J 7:41-8CrossRef
  Viswanath B, Chandra MS, Pallavi H, Reddy R (2008) Screening and assessment of laccase producing bacteria isolated from different environmental samples. Afr J Biotechnol 7:1129-133
  
• 作者单位：G. Nayanashree (1)
  B. Thippeswamy (1) (2)
  
  1. Department of P.G. Studies and Research in Microbiology, Bio-Science Complex, Jnanasahyadri, Kuvempu University, Shankaraghatta, Shivamogga (Dist.,), Karnataka, 577 451, India
  2. Visiting Scholar, Department of Plant Pathology, North Carolina State University, Raleigh, NC, USA
  
• 刊物类别：Environmental Science and Engineering; Environmental Management; Waste Management/Waste Technology;
• 刊物主题：Environmental Science and Engineering; Environmental Management; Waste Management/Waste Technology; Water Quality/Water Pollution;
• 出版者：Springer International Publishing
• ISSN：2198-7505

文摘

The present study was carried out to isolate rubber degrading bacteria and to check the enzymes responsible for degradation, so that the isolates can be effectively used in rubber degradation. Different bacteria were isolated from soil samples and natural rubber samples collected from a rubber processing unit, and were screened for their ability to degrade natural rubber by plate assay. A biodegradation experiment was carried out in the laboratory to confirm degradation. Enzymes responsible for natural rubber degradation were characterized, crude enzyme was extracted by submerged fermentation process and its activity was determined. After extraction of crude enzyme, it was partially purified by dialysis process, and then, the enzyme activity and specific activity were determined. Then, the ability of these enzymes to degrade natural rubber discs were checked. The ability of natural rubber degrading microorganisms to degrade rubber products such as rubber gloves discs and rubber tire discs, was tested. Among the isolated bacterial species, Bacillus subtilis was predominant and showed growth on the natural rubber discs. The biodegradation experiment showed 48.6 % weight loss. It was screened that laccase and manganese peroxidase enzymes are responsible for natural rubber degradation. When enzyme activity was determined, Bacillus subtilis, showed more manganese peroxidase activity compared to laccase activity. Laccase enzyme activity was 0.0138 IU and manganese peroxidase activity was 0.0155 IU. The molecular weights of laccase enzyme (64 kDa) and of manganese peroxidase enzyme (68 kDa) were determined. Biodegradation of rubber by microorganisms was confirmed by Schiff’s staining, SEM, FTIR and NMR studies. Keywords Bacillus subtilis Hevea brasiliensis Vulcanization Polyisoprene Laccase Manganese peroxidase