Degradation and detoxification of the triphenylmethane dye malachite green catalyzed by crude manganese peroxidase from Irpex lacteus F17
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- 作者：Xueting Yang ; Jinzhao Zheng ; Yongming Lu…
- 刊名：Environmental Science and Pollution Research
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：23
- 期：10
- 页码：9585-9597
- 全文大小：745 KB
- 刊物类别：Earth and Environmental Science
- 刊物主题：Environment
Environment
Atmospheric Protection, Air Quality Control and Air Pollution
Waste Water Technology, Water Pollution Control, Water Management and Aquatic Pollution
Industrial Pollution Prevention - 出版者：Springer Berlin / Heidelberg
- ISSN：1614-7499
- 卷排序：23
文摘
Malachite green (MG), a recalcitrant, carcinogenic, and mutagenic triphenylmethane dye, was decolorized and detoxified using crude manganese peroxidase (MnP) prepared from the white rot fungus Irpex lacteus F17. In this study, the key factors (pH, temperature, MG, Mn2+, H2O2, MnP) in these processes were investigated. Under optimal conditions, 96 % of 200 mg L−1 of MG was decolorized when 66.32 U L−1 of MnP was added for 1 h. The Km, Vmax, and kcat values were 109.9 μmol L−1, 152.8 μmol L−1 min−1, and 44.5 s−1, respectively. The decolorization of MG by MnP followed first-order reaction kinetics with a kinetic rate constant of 0.0129 h−1. UV–vis and UPLC analysis revealed degradation of MG. Furthermore, seven different intermediates formed during the MnP treatment of 0.5 h were identified by LC-TOF-MS. These degradation products were generated via two different routes by either N-demethylation of MG or the oxidative cleavage of the C–C double bond in MG. Based on ecotoxicity analyses performed on bacteria and algae, it was confirmed that MG metabolites produced by the MnP-catalyzed system were appreciably less toxic than the parent compound. These studies indicate the potential use of this enzyme system in the clean-up of aquatic and terrestrial environments.KeywordsMalachite greenManganese peroxidaseEnzymatic decolorizationKineticsDegradationEcotoxicity