Proteomic analysis of non-small cell lung cancer tissue interstitial fluids
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- 作者：Shaomin Li (1)
Rui Wang (2)
Mingxin Zhang (3)
Lina Wang (4)
Shaoli Cheng (5) - 关键词：Proteomic ; Non ; small cell lung cancer ; Tissue interstitial fluid ; Peroxiredoxin 1
- 刊名：World Journal of Surgical Oncology
- 出版年：2013
- 出版时间：December 2013
- 年：2013
- 卷：11
- 期：1
- 全文大小：707KB
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Rui Wang (2)
Mingxin Zhang (3)
Lina Wang (4)
Shaoli Cheng (5)
1. Department of Thoracic Surgery, Second Affiliated Hospital, Medical School, Xi’an Jiaotong University, Xi’an, Shaanxi Province, 710004, China
2. Department of Respiratory Medicine, Xi’an Central Hospital, Xi’an, Shaanxi Province, 710003, China
3. Department of Gastroenterology, Tangdu Hospital, Fourth Military Medical University, Xi’an, Shaanxi Province, 710038, China
4. Department of Emergency, Second Affiliated Hospital, Medical School, Xi’an Jiaotong University, Xi’an, Shaanxi Province, 710004, China
5. Morphological Experiment Centre, Medicine School, Xi’an Jiaotong University, Xia’an, Shaanxi Province, 710061, China
文摘
Background Non-small cell lung cancer (NSCLC) accounts for more than 80% of all lung cancers, and reliable biomarkers are desirable. The present investigation assesses our ability to identify tumor relevant proteins from NSCLC tissue interstitial fluid (TIF). Methods Paired TIF was collected from three NSCLC patients at the time of surgery, and resolved by two-dimensional gel electrophoresis and in-gel digestion for proteomic analysis. Differentially expressed spots were extracted from the two-dimensional gel and characterized by high-performance liquid chromatography-tandem mass spectrometry. Then, ELISA was used to verify the expression of peroxiredoxin 1 (PRDX1) in TIF of patients with NSCLC and benign lung disease. Finally, the relationship between expression of PRDX1 and clinicopathological features was determined. Results Comparative proteomic analysis showed 24 protein spots were differentially expressed with significant changes, including 11 upregulated proteins and 13 downregulated proteins. Of these, PRDX1 was selected for validation in TIF by Western blot and expression of PRDX1 was confirmed to be upregulated in tumor TIF. It was also demonstrated that PRDX1 was significantly elevated in 40 NSCLC patients with a mean level of 36.0?ng/mL compared to 6.26?ng/mL from 20 patients with benign lung disease. A significant correlation was found between the high level of PRDX1 expression and lymph node metastasis and tumor differentiation. Conclusions PRDX1 might be correlated with lymph node metastasis and differentiation, and its elevated expression in TIF may be an adverse biomarker for patients with NSCLC. PRDX1 may be attributed to the malignant transformation of NSCLC, and attention should be paid to a possible target for therapy.