Subtle genetic changes enhance virulence of methicillin resistant and sensitive Staphylococcus aureus
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- 作者：Sarah K Highlander (1) (3)
Kristina G Hultén (2)
Xiang Qin (3)
Huaiyang Jiang (3)
Shailaja Yerrapragada (3)
Edward O Mason Jr (2)
Yue Shang (3)
Tiffany M Williams (3)
Régine M Fortunov (2)
Yamei Liu (6)
Okezie Igboeli (3)
Joseph Petrosino (1)
Madhan Tirumalai (6)
Akif Uzman (5)
George E Fox (6)
Ana Maria Cardenas (1)
Donna M Muzny (3)
Lisa Hemphill (3)
Yan Ding (3)
Shannon Dugan (3)
Peter R Blyth (3)
Christian J Buhay (3)
Huyen H Dinh (3)
Alicia C Hawes (3)
Michael Holder (3)
Christie L Kovar (3)
Sandra L Lee (3)
Wen Liu (3)
Lynne V Nazareth (3)
Qiaoyan Wang (3)
Jianling Zhou (3)
Sheldon L Kaplan (2)
George M Weinstock (1) (3) (4) - 刊名：BMC Microbiology
- 出版年：2007
- 出版时间：December 2007
- 年：2007
- 卷：7
- 期：1
- 全文大小：984KB
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Kristina G Hultén (2)
Xiang Qin (3)
Huaiyang Jiang (3)
Shailaja Yerrapragada (3)
Edward O Mason Jr (2)
Yue Shang (3)
Tiffany M Williams (3)
Régine M Fortunov (2)
Yamei Liu (6)
Okezie Igboeli (3)
Joseph Petrosino (1)
Madhan Tirumalai (6)
Akif Uzman (5)
George E Fox (6)
Ana Maria Cardenas (1)
Donna M Muzny (3)
Lisa Hemphill (3)
Yan Ding (3)
Shannon Dugan (3)
Peter R Blyth (3)
Christian J Buhay (3)
Huyen H Dinh (3)
Alicia C Hawes (3)
Michael Holder (3)
Christie L Kovar (3)
Sandra L Lee (3)
Wen Liu (3)
Lynne V Nazareth (3)
Qiaoyan Wang (3)
Jianling Zhou (3)
Sheldon L Kaplan (2)
George M Weinstock (1) (3) (4)
1. Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, USA
3. Human Genome Sequencing Center, Baylor College of Medicine, Houston, TX, USA
2. Department of Pediatrics, Baylor College of Medicine, Texas Children's Hospital, Houston, TX, USA
6. University of Houston, Houston, TX, USA
5. University of Houston-Downtown, Houston, TX, USA
4. Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA - ISSN：1471-2180
文摘
Background Community acquired (CA) methicillin-resistant Staphylococcus aureus (MRSA) increasingly causes disease worldwide. USA300 has emerged as the predominant clone causing superficial and invasive infections in children and adults in the USA. Epidemiological studies suggest that USA300 is more virulent than other CA-MRSA. The genetic determinants that render virulence and dominance to USA300 remain unclear. Results We sequenced the genomes of two pediatric USA300 isolates: one CA-MRSA and one CA-methicillin susceptible (MSSA), isolated at Texas Children's Hospital in Houston. DNA sequencing was performed by Sanger dideoxy whole genome shotgun (WGS) and 454 Life Sciences pyrosequencing strategies. The sequence of the USA300 MRSA strain was rigorously annotated. In USA300-MRSA 2658 chromosomal open reading frames were predicted and 3.1 and 27 kilobase (kb) plasmids were identified. USA300-MSSA contained a 20 kb plasmid with some homology to the 27 kb plasmid found in USA300-MRSA. Two regions found in US300-MRSA were absent in USA300-MSSA. One of these carried the arginine deiminase operon that appears to have been acquired from S. epidermidis. The USA300 sequence was aligned with other sequenced S. aureus genomes and regions unique to USA300 MRSA were identified. Conclusion USA300-MRSA is highly similar to other MRSA strains based on whole genome alignments and gene content, indicating that the differences in pathogenesis are due to subtle changes rather than to large-scale acquisition of virulence factor genes. The USA300 Houston isolate differs from another sequenced USA300 strain isolate, derived from a patient in San Francisco, in plasmid content and a number of sequence polymorphisms. Such differences will provide new insights into the evolution of pathogens.