Engineering resistance to phage GVE3 in Geobacillus thermoglucosidasius

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• 作者：Leonardo Joaquim van Zyl ; Mark Paul Taylor…
• 关键词：Bacteriophage ; Geobacillus ; Resistance ; Polysaccharide pyruvyl transferase ; Immunity
• 刊名：Applied Microbiology and Biotechnology
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：100
• 期：4
• 页码：1833-1841
• 全文大小：880 KB
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• 作者单位：Leonardo Joaquim van Zyl (1)
  Mark Paul Taylor (2)
  Marla Trindade (1)
  
  1. Institute for Microbial Biotechnology and Metagenomics (IMBM), University of the Western Cape, Robert Sobukwe Road, Bellville, Cape Town, South Africa
  2. TMO Renewables Limited, 40 Alan Turing Road, The Surrey Research Park, Guildford, Surrey, GU2 7YF, UK
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Microbiology
  Microbial Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0614

文摘

Geobacillus thermoglucosidasius is a promising platform organism for the production of biofuels and other metabolites of interest. G. thermoglucosidasius fermentations could be subject to bacteriophage-related failure and financial loss. We develop two strains resistant to a recently described G. thermoglucosidasius-infecting phage GVE3. The phage-encoded immunity gene, imm, was overexpressed in the host leading to phage resistance. A phage-resistant mutant was isolated following expression of a putative anti-repressor-like protein and phage challenge. A point mutation was identified in the polysaccharide pyruvyl transferase, csaB. A double crossover knockout mutation of csaB confirmed its role in the phage resistance phenotype. These resistance mechanisms appear to prevent phage DNA injection and/or lysogenic conversion rather than just reducing efficiency of plating, as no phage DNA could be detected in resistant bacteria challenged with GVE3 and no plaques observed even at high phage titers. Not only do the strains developed here shed light on the biological relationship between the GVE3 phage and its host, they could be employed by those looking to make use of this organism for metabolite production, with reduced occurrence of GVE3-related failure. Keywords Bacteriophage Geobacillus Resistance Polysaccharide pyruvyl transferase Immunity