Recovery of Phenolic Compounds from Eucalyptus globulus Wood Wastes using PEG/phosphate Aqueous Two-Phase Systems

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• 作者：Lucía Xavier ; M. Sonia Freire ; Isabel Vidal-Tato…
• 关键词：Aqueous two ; phase systems ; Eucalyptus wood ; Biomass wastes ; Extraction ; Phenolic compounds
• 刊名：Waste and Biomass Valorization
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：8
• 期：2
• 页码：443-452
• 全文大小：
• 刊物类别：Engineering
• 刊物主题：Engineering, general; Environment, general; Renewable and Green Energy; Industrial Pollution Prevention; Waste Management/Waste Technology; Environmental Engineering/Biotechnology;
• 出版者：Springer Netherlands
• ISSN：1877-265X
• 卷排序：8

文摘

Polyethylene glycol (PEG) 2000/phosphate salt based aqueous two-phase systems (ATPS) were used to recover phenolic compounds with antioxidant activity from wastes of eucalyptus wood veneers. Combinations of two phosphate salts (K₂HPO₄ or mixtures of K₂HPO₄ and KH₂PO₄) with PEG 2000 were studied in order to understand the influence of pH and type of salt on phenols partition. Each ATPS was studied under different conditions (polymer/salt ratio, temperature, volume ratio and solid–liquid ratio). In all the ATPS studied, it was found that phenols had preference for the PEG-rich phase and it was demonstrated that the highest phenols recovery was favored by a high polymer/salt ratio, high temperature and a high volume ratio. The maximum total phenols yield in the top phase, 1.89 mg gallic acid equivalent/100 mg oven dried wood, was attained at 65 °C and a solid/liquid ratio of 0.5:10 using a PEG 2000/K₂HPO₄ ATPS. However, higher partition coefficients were attained for PEG 2000/K₂HPO₄–KH₂PO₄ ATPS. It was found that at low pH values higher partition of phenolic compounds was achieved. Extract analysis by RP-HPLC–ESI-TOF confirmed the presence of various phenolics compounds with antioxidant activity such as ellagic acid and quercetin 3-O-rhamnoside.