Effect of a nerve graft substitute single-walled carbon nanotubes on rat pheochromocytoma cells

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• 作者：Qian Wu ; Juan Zhang ; Zheqin Hu
• 关键词：nerve graft substitute ; single ; walled carbon nanotubes (SWNTs) ; oxidative stress ; PC12 cells
• 刊名：Journal of Wuhan University of Technology--Materials Science Edition
• 出版年：2015
• 出版时间：August 2015
• 年：2015
• 卷：30
• 期：4
• 页码：847-851
• 全文大小：643 KB
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• 作者单位：Qian Wu (1)
  Juan Zhang (1)
  Zheqin Hu (1)
  
  1. Maternal and Child Health Hospital of Hubei, Wuhan, 430070, China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Materials Science
  Chinese Library of Science
  
• 出版者：Wuhan University, co-published with Springer
• ISSN：1993-0437

文摘

Carbon nanotubes (CNTs) were extensively explored for their beneficial use in nervous system tissue engineering. However, an important concern regarding the use of CNTs is their toxicity during the interaction between cells and the nano particles. The rat pheochromocytoma cell line (PC12) was co-cultured with three types of single-walled carbon nanotubes (SWNTs), purified raw SWNTs (C), hydroxyl purified SWNTs (C-OH) and carboxyl purified SWNTs (C-COOH) at 25 μg/mL and 100 μg/ml. The experimental results revealed that SWNTs at the concentration below 100 μg/mL did not affect the cell viability. Notably, powerful antioxidant system in nerous system tissue is able to counteract with the toxicity of CNTs, which is characterized by the prominently enhanced expression of main antioxidant enzymes (catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione-S-transferase (GST)). Therefore, we believe that CNTs can be good candidates for the fabrication of biomedical scaffolds for the nerve tissue repair.