Using Amplified Fragment Length Polymorphisms (AFLP) to Study Genetic Variability in Several Freshwater Rotifer Species

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• 作者：Sanjuana Hern谩ndez-Delgado ; Netzahualcoyotl Mayek-P茅rez ; Gustavo Emilio Santos-Medrano and Roberto Rico-Mart铆nez
• 关键词：DNA ; genetic diversity ; AFLP ; phylogeny ; Rotifera
• 刊名：Hydrobiologia
• 出版年：2005
• 出版时间：September, 2005
• 年：2005
• 卷：546
• 期：1
• 页码：109-115
• 全文大小：305.2 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Hydrobiology
  Ecology
  
• 出版者：Springer Netherlands
• ISSN：1573-5117

文摘

We have used amplified fragment length polymorphisms (AFLP) to investigate the potential of this technique as a tool to measure genetic variability in eight species of freshwater rotifers: Brachionus calyciflorus, Lecane bulla, L. luna, L. quadridentata, Plationus patulus, Philodina acuticornis odiosa, Rotaria neptunia, and R. rotatoria. We used nine combinations of oligonucleotides. We observed a total of 806 amplified bands, 798 polymorphic and 8 monomorphic. The data were analyzed using cluster analysis with UPGMA, first within each set of oligonucleotide combination and finally using all nine combinations. Our best dendrogram clearly separated monogononts from digononts, and grouped the species of monogononts in the two genera. However, it grouped R. neptunia with P. acuticornis odiosa rather than with R. rotatoria. These results are discussed in view of recent works in the literature measuring genetic variability and discussing the phylogeny of the Rotifera.