GmPAP4, a novel purple acid phosphatase gene isolated from soybean (Glycine max), enhanced extracellular phytate utilization in Arabidopsis thaliana

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• 作者：Youbin Kong (1)
  Xihuan Li (1)
  Jun Ma (2)
  Wenlong Li (1)
  Guijun Yan (1) (2)
  Caiying Zhang (1)
  
• 关键词：Soybean (Glycine max) ; Purple acid phosphatase ; Phytate ; Phosphorus utilization
• 刊名：Plant Cell Reports
• 出版年：2014
• 出版时间：April 2014
• 年：2014
• 卷：33
• 期：4
• 页码：655-667
• 全文大小：4,804 KB
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• 作者单位：Youbin Kong (1)
  Xihuan Li (1)
  Jun Ma (2)
  Wenlong Li (1)
  Guijun Yan (1) (2)
  Caiying Zhang (1)
  
  1. North China Key Laboratory for Germplasm Resources of Education Ministry, Department of Plant Genetics and Breeding, Hebei Agricultural University, Baoding, 071001, People’s Republic of China
  2. School of Plant Biology, Faculty of Science and UWA Institute of Agriculture, The University of Western Australia, Perth, WA, 6009, Australia
  
• ISSN：1432-203X

文摘

Key message GmPAP4 , a novel plant PAP gene in soybean, has phytase activity. Over-expressing GmPAP4 can enhance Arabidopsis growth when phytate is the sole P source in culture. Abstract Phosphorus (P) is an important macronutrient for plant growth and development. However, most of the total P in soils is fixed into organic phosphate (Po). Purple acid phosphatase (PAP) can hydrolyze Po in the soil to liberate inorganic phosphate and enhance plant P utilization. We isolated a novel PAP gene, GmPAP4, from soybean (Glycine max). It had an open reading frame of 1,329?bp, encoding 442 amino acid residues. Sequence alignment and phylogenetics analysis indicated that GmPAP4 was similar to other plant PAPs with large molecular masses. Quantitative real-time PCR analysis showed that the induced expression of GmPAP4 was greater in P-efficient genotype Zhonghuang15 (ZH15) than in P-inefficient genotype Niumaohuang (NMH) during the periods of flowering (28-5?days post phytate stress; DPP) and pod formation (49-3 DPP). Moreover, peak expression, at 63 DPP, was about 3-fold higher in ‘ZH15-than in ‘NMH- Sub-cellular localization showed that GmPAP4 might be on plasma membrane or in cytoplasm. Over-expressing GmPAP4 in Arabidopsis resulted in significant rises in P acquisition and utilization compared with the wild-type (WT). Under phytate condition, transgenic Arabidopsis plants showed increases of approximately 132.7?% in dry weight and 162.6?% in shoot P content compared with the WT. Furthermore, when phytate was added as the sole P source in cultures, the activity of acid phosphatase was significantly higher in transgenic plants. Therefore, GmPAP4 is a novel PAP gene that functions in plant’s utilization of organic phosphate especially under phytate condition.