Modification of targets related to the Entner–Doudoroff/pentose phosphate pathway route for methyl-d-erythritol 4-phosphate-dependent carotenoid biosynthesis in Escherich
详细信息 查看全文
- 作者：Chun Li ; Lan-Qing Ying ; Sha-Sha Zhang ; Nan Chen ; Wei-Feng Liu…
- 关键词：Carotenoid biosynthesis ; Methyl ; d ; erythritol 4 ; phosphate pathway ; Genetic targets ; Central metabolic pathways ; Escherichia coli ; Balance of precursors
- 刊名：Microbial Cell Factories
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：14
- 期：1
- 全文大小：1,985 KB
- 刊物类别：Chemistry and Materials Science
- 刊物主题：Biotechnology
Applied Microbiology
Environmental Engineering/Biotechnology - 出版者：BioMed Central
- ISSN：1475-2859
文摘
Background In engineered strains of Escherichia coli, bioconversion efficiency is determined by not only metabolic flux but also the turnover efficiency of relevant pathways. Methyl-d-erythritol 4-phosphate (MEP)-dependent carotenoid biosynthesis in E. coli requires efficient turnover of precursors and balanced flux among precursors, cofactors, and cellular energy. However, the imbalanced supply of glyceraldehyde 3-phosphate (G3P) and pyruvate precursors remains the major metabolic bottleneck. To address this problem, we manipulated various genetic targets related to the Entner–Doudoroff (ED)/pentose phosphate (PP) pathways. Systematic target modification was conducted to improve G3P and pyruvate use and rebalance the precursor and redox fluxes.