Calcium phosphate nanoparticles as versatile carrier for small and large molecules across cell membranes

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• 作者：Viktoriya Sokolova (1)
  Olga Rotan (1)
  Jan Klesing (1)
  Perihan Nalbant (2)
  Jan Buer (3)
  Torben Knuschke (3)
  Astrid M. Westendorf (3)
  Matthias Epple (1) matthias.epple@uni-due.de
• 关键词：Calcium phosphate &#8211 ; Drug delivery &#8211 ; Cells &#8211 ; Nanomedicine
• 刊名：Journal of Nanoparticle Research
• 出版年：2012
• 出版时间：June 2012
• 年：2012
• 卷：14
• 期：6
• 页码：DOI: 10.1007/s11051-
• 全文大小：567.5 KB
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• 作者单位：1. Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, Universitaetsstr. 5-7, 45117 Essen, Germany2. Faculty of Biology, Institute of Molecular Cell Biology, University of Duisburg-Essen, 45117 Essen, Germany3. Institute of Medical Microbiology, University Hospital Essen, University of Duisburg-Essen, Hufelandstr. 55, 45147 Essen, Germany
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Nanotechnology
  Inorganic Chemistry
  Characterization and Evaluation Materials
  Physical Chemistry
  Applied Optics, Optoelectronics and Optical Devices
  
• 出版者：Springer Netherlands
• ISSN：1572-896X

文摘

The successful transport of molecules across the cell membrane is a key point in biology and medicine. In most cases, molecules alone cannot penetrate the cell membrane, therefore an efficient carrier is needed. Calcium phosphate nanoparticles (diameter: 100–250 nm, depending on the functionalization) were loaded with fluorescent oligonucleotides, peptide, proteins, antibodies, polymers or porphyrins and characterized by dynamic light scattering, nanoparticle tracking analysis and scanning electron microscopy. Any excess of molecules was removed by ultracentrifugation, and the dissolved molecules at the same concentration were used as control. The uptake of such fluorescence-labeled nanoparticles into HeLa cells was monitored by fluorescence microscopy and confocal laser scanning microscopy. Calcium phosphate nanoparticles were able to transport all molecules across the cell membrane, whereas the dissolved molecules alone were taken up only to a very small extent or even not at all.