Microsatellite-aided detection of genetic redundancy improves management of the International Cocoa Genebank, Trinidad

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• 作者：Lambert A. Motilal (1)
  Dapeng Zhang (2)
  Sue Mischke (2)
  Lyndel W. Meinhardt (2)
  Pathmanathan Umaharan (1)
  
• 关键词：Cocoa ; Conservation management ; Sibship ; Core collection ; Field genebank ; Redundancy
• 刊名：Tree Genetics & Genomes
• 出版年：2013
• 出版时间：December 2013
• 年：2013
• 卷：9
• 期：6
• 页码：1395-1411
• 全文大小：
• 作者单位：Lambert A. Motilal (1)
  Dapeng Zhang (2)
  Sue Mischke (2)
  Lyndel W. Meinhardt (2)
  Pathmanathan Umaharan (1)
  
  1. Cocoa Research Centre, The University of the West Indies, St. Augustine, Trinidad, Republic of Trinidad and Tobago
  2. Beltsville Agricultural Research Center, SPCL, USDA/ARS, 10300 Baltimore Avenue, Bldg. 001, Rm. 223, BARC-W, Beltsville, MD, 20705, USA
  
• ISSN：1614-2950

文摘

Cacao (Theobroma cacao L.), the tree from which cocoa butter and chocolate is derived, is conserved in field genebanks. The largest of these ex situ collections in the public domain is the International Cocoa Genebank, Trinidad (ICG,T). Reduction of genetic redundancy is essential to improve the accuracy and efficiency of genebank management. This study examined the pedigree and genetic diversity in a subset of 387 accessions in this collection. Sibship reconstruction of this subset revealed 56 full-sib families nested within 189 half-sib families. Sixteen centers of interconnectivity were identified, which suggested a high level of genetic redundancy in the collection. Generally, consistent phylogenetic trees were obtained using different genetic distance measures. However, a principal coordinate analysis of the D est differentiation measure elicited the best representation of accession group clustering, and we recommend this approach when probing fine-scale genetic differentiation among cacao accessions. The composite genetic diversity of 414 cacao accessions was contained in a core set of 59 unique accessions. These results have significant implications in the conservation of genetic resources of the ICG,T and other cacao genebanks. The approach developed in this study is recommended as a strategy to curators in guiding conservation management practices of cacao and other similar ex situ genebanks.