Phytic acid dynamics during seed development and it’s composition in yellow and black Indian soybean (Glycine max L.) genotypes through a modified extraction and HPLC method

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• 作者：Vanita Pandey ; Veda Krishnan…
• 关键词：Phytic acid ; HPLC ; Dynamics ; Black soybean ; Anti ; nutrient ; Nutraceutical
• 刊名：Journal of Plant Biochemistry and Biotechnology
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：25
• 期：4
• 页码：367-374
• 全文大小：568 KB
• 刊物主题：Life Sciences, general; Plant Biochemistry; Protein Science; Receptors; Cell Biology;
• 出版者：Springer India
• ISSN：0974-1275
• 卷排序：25

文摘

Identifying or designing low phytic acid soybean demands an improved understanding of its dynamics and a need for a reliable and accurate phytate quantification method and hence an improved quantitative technique for accurate and rapid quantification of phytic acid (PA) using high-performance liquid chromatography is proposed in this paper. A rapid PA extraction method utilizing sonication of sample in 0.78 M HCl for 3 min followed by mechanical agitation and separation using strong anion exchange column in a vacuum manifold was optimized. The elution of PA was performed using a RP-C₁₈ column with an isocratic mobile phase [Acetonitrile, 35 mM formic acid and tetrabutylammonium hydroxide (4.8: 5.1: 0.1, v/v/v)]. The modified method was rapid, accurate, precise, and reproducible with relative standard deviation of 1.80 and 3.01 % (n = 10, for 1 mg ml−1) for within and between days respectively with linearity (R2 = 0.999, P < 0.05), low limit of detection (LOD = 7.8 μg ml−1) and limit of quantification (LOQ = 31.25 μg ml−1). PA dynamics was found increased in a linear trend from initial to later developing stages until maturity in both yellow (DS-9814) and black (DS-MM-64) genotypes. The PA content ranged from 2.38–4.72 g 100g−1 in the 20 soybean genotypes screened and the variability in PA content was more in black genotypes (P < 0.0001). The black soybean genotypes JS 76-205 (2.38 g 100g−1), Kalitur (2.50 g 100g−1) and UPSL 652 (2.54 g 100g−1), inherently rich in anthocyanin, contains the lowest PA content and hence can serve as potential genotypes with nutraceutical benefits.