Differential proteomics of Picrorhiza kurrooa Royle ex Benth. in response to dark stress

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• 作者：Jai Parkash ; Sanjeeta Kashyap ; Prakash Jyoti Kalita…
• 关键词：Picrorhiza ; Proteomics ; Mass spectrometry ; MALDI ; ToF/ToF ; Dark stress
• 刊名：Molecular Biology Reports
• 出版年：2014
• 出版时间：September 2014
• 年：2014
• 卷：41
• 期：9
• 页码：6051-6062
• 全文大小：671 KB
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• 作者单位：Jai Parkash (1) (2)
  Sanjeeta Kashyap (1)
  Prakash Jyoti Kalita (1) (3)
  Manjula Devi (1)
  Paramvir Singh Ahuja (1) (2)
  Som Dutt (1) (2)
  
  1. Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology (CSIR-IHBT), Palampur, 176061, Himachal Pradesh, India
  2. Academy of Scientific and Innovative Research (AcSIR), New Delhi, India
  3. Division of Bioscience and Bioinformatics, College of Natural Science, Myongi University, San 38-2 Namdong, Cheoin-Gu, Yongin, Gyeonggido, 449-728, South Korea
  
• ISSN：1573-4978

文摘

Picrorhiza (Picrorhiza kurrooa Royle ex Benth.) an important medicinal herb of western Himalayan region has been used to treat various diseases and disorders. Over-harvesting and lack of cultivation has led to its entry in Red Data Book as an endangered species. Further, its very restrictive habitat and lesser biomass production are major limitations for bringing it under commercial cultivation. All these issues necessitate deeper insights into mechanisms governing its growth and interaction with the environmental cues. Light may be one of the important factors to be studied for its role in regulating growth and adaptation of Picrorhiza as in natural habitat it prefers shady niches. Keeping this in view, proteome of Picrorhiza kept under light vis-à-vis under dark was analysed and compared. Leaf as well as root proteome of Picrorhiza was studied. Denaturing two dimensional gel electrophoresis and mass spectrometry techniques were used to detect and identify differentially expressed proteins, respectively. Twenty two proteins from leaf and 25 proteins from root showed differential expression levels under dark and light conditions. Among the differentially expressed proteins, majority were those involved in metabolism, protein synthesis, and stress and defense response. Other differentially expressed proteins were those involved in photosynthetic process, photorespiration and few proteins were with unknown function indicating that many different processes work together to establish a new cellular homeostasis in response to dark and light conditions. Proteins found to be differentially expressed under light vis-à-vis dark conditions suggested a range of biochemical pathways and processes being associated with response of plant to dark conditions. The identified proteins may be utilized for developing strategies for improving the biomass production/performance of Picrorhiza under varied light/dark habitats.