Characterisation of secretory calcium-binding phosphoprotein-proline-glutamine-rich 1: a novel basal lamina component expressed at cell-tooth interfaces

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• 作者：Pierre Moffatt (1) (2)
  Rima M. Wazen (3)
  Juliana Dos Santos Neves (3)
  Antonio Nanci (3) (4)
  
• 关键词：Basal lamina ; Enamel organ ; Junctional epithelium ; Secretory calcium ; binding phosphoprotein ; proline ; glutamine ; rich 1 ; Rat ; Mouse
• 刊名：Cell and Tissue Research
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：358
• 期：3
• 页码：843-855
• 全文大小：4,106 KB
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• 作者单位：Pierre Moffatt (1) (2)
  Rima M. Wazen (3)
  Juliana Dos Santos Neves (3)
  Antonio Nanci (3) (4)
  
  1. Shriners Hospital for Children, Montr茅al, Qu茅bec, Canada
  2. Department of Human Genetics, McGill University, Montr茅al, Qu茅bec, Canada
  3. Laboratory for the Study of Calcified Tissues and Biomaterials, Department of Stomatology, Faculty of Dentistry, Universit茅 de Montr茅al, Montr茅al, Qu茅bec, Canada
  4. Department of Biochemistry and Molecular Medicine, Universit茅 de Montr茅al, Montr茅al, Qu茅bec, Canada
  
• ISSN：1432-0878

文摘

Functional genomic screening of the rat enamel organ (EO) has led to the identification of a number of secreted proteins expressed during the maturation stage of amelogenesis, including amelotin (AMTN) and odontogenic ameloblast-associated (ODAM). In this study, we characterise the gene, protein and pattern of expression of a related protein called secretory calcium-binding phosphoprotein-proline-glutamine-rich 1 (SCPPPQ1). The Scpppq1 gene resides within the secretory calcium-binding phosphoprotein (Scpp) cluster. SCPPPQ1 is a highly conserved, 75-residue, secreted protein rich in proline, leucine, glutamine and phenylalanine. In silico data mining has revealed no correlation to any known sequences. Northern blotting of various rat tissues suggests that the expression of Scpppq1 is restricted to tooth and associated tissues. Immunohistochemical analyses show that the protein is expressed during the late maturation stage of amelogenesis and in the junctional epithelium where it localises to an atypical basal lamina at the cell-tooth interface. This discrete localisation suggests that SCPPPQ1, together with AMTN and ODAM, participates in structuring the basal lamina and in mediating attachment of epithelia cells to mineralised tooth surfaces.