Molecular Cloning and Characterization of the Light-Harvesting Chlorophyll a/b Gene from the Pigeon pea (Cajanus cajan)

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• 作者：Guang Qiao ; Xiao-Peng Wen ; Ting Zhang
• 关键词：Pigeon pea (Cajanus cajan) ; Light ; harvesting chlorophyll a/b gene ; RACE ; Gene expression ; Bioinformatics analysis
• 刊名：Applied Biochemistry and Biotechnology
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：177
• 期：7
• 页码：1447-1455
• 全文大小：801 KB
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• 作者单位：Guang Qiao (1)
  Xiao-Peng Wen (1)
  Ting Zhang (1)
  
  1. Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region and Ministry of Education, Institute of Agro-bioengineering, Guizhou University, Guiyang, 550025, China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Biochemistry
  
• 出版者：Humana Press Inc.
• ISSN：1559-0291

文摘

Light-harvesting chlorophyll a/b-binding proteins (LHCB) have been implicated in the stress response. In this study, a gene encoding LHCB in the pigeon pea was cloned and characterized. Based on the sequence of a previously obtained 327 bp Est, a full-length 793 bp cDNA was cloned using the rapid amplification of cDNA ends (RACE) method. It was designated CcLHCB1 and encoded a 262 amino acid protein. The calculated molecular weight of the CcLHCB1 protein was 27.89 kDa, and the theoretical isoelectric point was 5.29. Homology search and sequence multi-alignment demonstrated that the CcLHCB1 protein sequence shared a high identity with LHCB from other plants. Bioinformatics analysis revealed that CcLHCB1 was a hydrophobic protein with three transmembrane domains. By fluorescent quantitative real-time polymerase chain reaction (PCR), CcLHCB1 mRNA transcripts were detectable in different tissues (leaf, stem, and root), with the highest level found in the leaf. The expression of CcLHCB1 mRNA in the leaves was up-regulated by drought stimulation and AM inoculation. Our results provide the basis for a better understanding of the molecular organization of LCHB and might be useful for understanding the interaction between plants and microbes in the future.