The pseudorabies virus DNA polymerase processivity factor UL42 exists as a monomer in vitro and in vivo

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• 作者：Yi-Ping Wang ; Li-Ping Huang ; Wen-Juan Du ; Yan-Wu Wei ; De-Li Xia…
• 关键词：Pseudorabies virus ; DNA polymerase ; Processivity factor ; UL42 ; Monomer
• 刊名：Archives of Virology
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：161
• 期：4
• 页码：1027-1031
• 全文大小：1,067 KB
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• 作者单位：Yi-Ping Wang (1)
  Li-Ping Huang (1)
  Wen-Juan Du (1)
  Yan-Wu Wei (1)
  De-Li Xia (1)
  Hong-Li Wu (1)
  Li Feng (1)
  Chang-Ming Liu (1)
  
  1. Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin, 150001, People’s Republic of China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Virology
  Medical Microbiology
  Infectious Diseases
  
• 出版者：Springer Wien
• ISSN：1432-8798

文摘

The processivity factors (PFs) of herpesviruses confer processivity to the DNA polymerase. Understanding whether the herpesvirus PFs function as monomers or multimers is important for clarifying the mechanism by which they provide the DNA polymerase with processivity. Herpes simplex virus type 1 UL42 is a monomer, whereas human cytomegalovirus UL44, Epstein-Barr virus BMRF1, and Kaposi’s sarcoma-associated herpesvirus PF-8 exist as dimers. However, the oligomeric status of the pseudorabies virus (PRV) DNA polymerase PF UL42 has not been determined. Using fluorescence confocal microscopy and chemical crosslinking, we confirmed that UL42 is a monomer when expressed in vitro. Crosslinking of nuclear extracts from PRV-infected or uninfected PK-15 cells verified that UL42 exists as a monomer in vivo. Our demonstration that UL42 exists as a monomer in vitro and in vivo contributes to the further investigation of the mechanism used by UL42 to achieve processivity.