The roles of IL-2 and IL-10 enhance anti-CD45RBmAb immune inhibition in allograft skin

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• 作者：Wei-Jian Guo (1)
  Hui Qi (1)
  Chun-Yan Deng (1)
  Han-Xin Zhou (2)
  Shao-Ping Deng (3)
  Fu-Rong Li (1) (2)
  
  1. The Key Laboratory of Stem Cell and Cellular Therapy ; the Second Clinical Medical College (Shenzhen People鈥檚 Hospital) ; Ji鈥檔an University ; Nu.1017 ; North Road of Eastern Gate ; Shenzhen ; 518020 ; China
  2. Shenzhen Institute of Gerontology ; Shenzhen ; 518020 ; China
  3. Department of Surgery ; Massachusetts General Hospital ; Harvard Medical School ; Boston ; MA ; USA
  
• 关键词：Anti ; CD45RB monoclonal antibodies ; Immune tolerance ; Skin allograft ; IL ; 2 ; IL ; 10
• 刊名：Immunologic Research
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：61
• 期：3
• 页码：250-259
• 全文大小：1,368 KB
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• 刊物主题：Allergology; Immunology; Medicine/Public Health, general; Internal Medicine;
• 出版者：Springer US
• ISSN：1559-0755

文摘

As a new type of immune tolerance inducer, anti-CD45RB monoclonal antibodies (anti-CD45RBmAb) can prolong the graft survival time of animal organs or cell transplantation as well as induce stable immune tolerance. Both interleukin (IL)-2 and IL-10 have important roles in the induction and maintenance of immunological tolerance. However, whether these cytokines combined with anti-CD45RBmAb can promote immune tolerance is poorly understood. Therefore, we investigated the effect of IL-2 and IL-10 in vitro and in vivo on the tolerance induction by anti-CD45RBmAb. The changes of Treg and Th17 cells and Th1/Th2 cytokines in anti-CD45RBmAb induced prolongation of skin allograft survival in mice. The finding of a role for IL-2 is novel, of interest, IL-2 promoted anti-CD45RBmAb-induced CD4+ T cell differentiation into Treg and Th2 cells and suppressed Th17 and Th1 cells. IL-2 enhanced the induction of immune tolerance by anti-CD45RBmAb and significantly prolonged skin graft survival time in vivo. In contrast, this effect should be demonstrated experimentally by neutralizing IL-2 and inhibition of the effect of anti-CD45RBmAb, and neutralizing IL-10 showed no effect for anti-CD45RBmAb-induced tolerance. These data reveal that IL-2 significantly enhances anti-CD45RBmAb-induced immune tolerance via up-regulated T regulatory (Treg) cells and the balance of Th1/Th2 shifts. Conversely, IL-10 showed no effect on anti-CD45RBmAb-induced tolerance.