Construction of allitol synthesis pathway by multi-enzyme coexpression in Escherichia coli and its application in allitol production

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• 作者：Yueming Zhu ; Hongyi Li ; Pingping Liu…
• 关键词：Allitol ; Multi ; enzyme coupling pathway ; Cofactor recycling system ; Whole ; cell biotransformation
• 刊名：Journal of Industrial Microbiology and Biotechnology
• 出版年：2015
• 出版时间：May 2015
• 年：2015
• 卷：42
• 期：5
• 页码：661-669
• 全文大小：708 KB
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  3. Chandrasekhar B (2010) Synthesis of some monocyclic and bicyclic polyhydroxy nitrogen heterocyclic compounds (Azasugars). PhD Thesis, Indian Institute of Chemical Technology, Hyderabad
  4. Han, W, Zhu, Y, Men, Y, Yang, J, Liu, C, Sun, Y (2014) Production of allitol from d-psicose by a novel isolated strain of Klebsiella oxytoca G4A4. J Basic Microbiol 53: pp. 1-7
  5. Ishida, Y, Kamiya, T, Itoh, H, Kimura, Y, Izumori, K (1997) Cloning and characterization of the d-tagatose 3-epimerase gene from Pseudomonas cichorii ST-24. J Ferment Bioeng 83: pp. 529-534 CrossRef
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  10. Kim, HJ, Hyun, EK, Kim, YS, Lee, YJ, Oh, DK (2006) Characterization of an Agrobacterium tumefaciens d-psicose 3-epimerase that converts d-fructose to d-psicose. Appl Environ Microbiol 72: pp. 981-985 CrossRef
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• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Chemistry
  Biotechnology
  Genetic Engineering
  Biochemistry
  Bioinformatics
  Microbiology
  Microbial Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1476-5535

文摘

An engineered strain for the conversion of d-fructose to allitol was developed by constructing a multi-enzyme coupling pathway and cofactor recycling system in Escherichia coli. d-Psicose-3-epimerase from Ruminococcus sp. and ribitol dehydrogenase from Klebsiella oxytoca were coexpressed to form the multi-enzyme coupling pathway for allitol production. The cofactor recycling system was constructed using the formate dehydrogenase gene from Candida methylica for continuous NADH supply. The recombinant strain produced 10.62?g/l allitol from 100?mM d-fructose. To increase the intracellular concentration of the substrate, the glucose/fructose facilitator gene from Zymomonas mobilis was incorporated into the engineered strain. The results showed that the allitol yield was enhanced significantly to 16.53?g/l with a conversion rate of 92?%. Through optimizing conversion conditions, allitol was produced effectively on a large scale by the whole-cell biotransformation system; the yield reached 48.62?g/l when 500?mM d-fructose was used as the substrate.