AcPIP2, a plasma membrane intrinsic protein from halophyte Atriplex canescens, enhances plant growth rate and abiotic stress tolerance when overexpressed in Arabidopsis thaliana

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• 作者：Jingtao Li ; Gang Yu ; Xinhua Sun ; Yanzhi Liu ; Jinliang Liu…
• 关键词：Aquaporin ; Atriplex canescens ; Overexpression ; Plant growth ; Abiotic stress
• 刊名：Plant Cell Reports
• 出版年：2015
• 出版时间：August 2015
• 年：2015
• 卷：34
• 期：8
• 页码：1401-1415
• 全文大小：2,843 KB
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• 作者单位：Jingtao Li (1)
  Gang Yu (1)
  Xinhua Sun (1)
  Yanzhi Liu (1)
  Jinliang Liu (1)
  Xianghui Zhang (1)
  Chengguo Jia (1)
  Hongyu Pan (1)
  
  1. College of Plant Sciences, Jilin University, Changchun, 130062, Jilin, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Plant Sciences
  Biotechnology
  Plant Biochemistry
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-203X

文摘

Key message An aquaporin protein AcPIP2 from Atriplex canescens was involved in plant growth rate, abiotic stress tolerance in Arabidopsis. Under limited water condition, AcPIP2 leaded to the sensitivity to drought stress. Abstract An aquaporin protein (AcPIP2) was obtained from the saltbush Atriplex canescens, which was in PIP2 subgroup belonging to the PIP subfamily, MIP superfamily. The subcellular localization of AcPIP2 showed the fusion protein AcPIP2-eGFP located at the plasma membrane in Nicotiana benthamiana. Overexpression of AcPIP2 in Arabidopsis fully proved that AcPIP2 was involved in plant growth rate, transpiration rate and abiotic stress tolerance (NaCl, drought and NaHCO3) in Arabidopsis, which is mostly in correspondence to gene expression pattern characterized by qRT-PCR performed in A. canescens. And under limited water condition, AcPIP2 overexpression leaded to the sensitivity to drought stress. In the view of the resistant effect in transgenic Arabidopsis overexpressing AcPIP2, the AcPIP2 may throw some light into understanding how the A. canescens plants cope with abiotic stress, and could be used in the genetic engineering to improve plant growth or selective tolerance to the abiotic stress.