Cloning PIP genes in drought-tolerant vetiver grass and responses of transgenic VzPIP2;1 soybean plants to water stress
详细信息 查看全文
- 作者：S. B. Hu ; Q. Zhou ; J. An ; B. J. Yu
- 关键词：Additional key wordsAgrobacterium ; Glycine max ; root hydraulic conductivity ; transpiration ; Vetiveria zizanioides
- 刊名：Biologia Plantarum
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：60
- 期：4
- 页码：655-666
- 全文大小：
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Plant Sciences;
- 出版者：Springer Netherlands
- ISSN：1573-8264
- 卷排序：60
文摘
Vetiver grass [Vetiveria zizanioides (L.) Nash] displays comprehensive abiotic stress tolerance closely related to fine maintenance of plant water relation mediated by plasma membrane intrinsic proteins (PIPs). Two open reading frame sequences of PIPs (867 and 873 bp) were cloned from vetiver grass and named as VzPIP1;1 and VzPIP2;1, respectively. Expression of green fluorescent protein revealed only subcellular localization of VzPIP2;1 in the plasma membrane. Agrobacterium tumefaciens mediated transgenic (VzPIP2;1) soybean plants had a higher water content in above-ground parts under sufficient water supply through enhancing transpiration as compared to the non-transgenic plants but displayed a more severe drought injury because of a lower photosynthesis and a higher transpiration rate. However, A. rhizogenes mediated transgenic soybean plants kept a higher water content in above-ground parts by improving root water transport and kept a more effective photosynthesis under normal and drought conditions.