Five pentaplex real-time PCR systems for the efficient determination of 20 genetically modified maize traits in food

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• 作者：René Köppel ; Arthika Ganeshan…
• 关键词：gm Maize ; Multiplex ; Real ; time quantitative PCR ; Validation
• 刊名：European Food Research and Technology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：243
• 期：2
• 页码：215-225
• 全文大小：
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Food Science; Analytical Chemistry; Biotechnology; Agriculture; Forestry;
• 出版者：Springer Berlin Heidelberg
• ISSN：1438-2385
• 卷排序：243

文摘

According to the EU and Swiss food legislation, only authorised traits of transgene plants are allowed to be imported and sold to the consumer. In order to control imports of maize products from retailers, efficient and reliable methods for the detection and quantification are a prerequisite. Actually, in the EU, more than 25 gm Maize traits were authorised and are allowed to be sold. To ensure correct labelling and exclude unauthorised gm traits from sale, five multiplex real-time polymerase chain reaction systems were developed and validated. These 20 systems for the most prevalent maize gm traits allow relative multiplex quantification and/or delta–delta Ct method quantification in routine in a reliable, cost- and time-efficient way.