Hydrogels for 3D mammalian cell culture: a starting guide for laboratory practice

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• 作者：Ferdinand Ruedinger (1)
  Antonina Lavrentieva (1)
  Cornelia Blume (1)
  Iliyana Pepelanova (1)
  Thomas Scheper (1)
  
• 关键词：Hydrogel ; 3D cell culture ; Mammalian cell culture ; 3D analytics ; Material design
• 刊名：Applied Microbiology and Biotechnology
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：99
• 期：2
• 页码：623-636
• 全文大小：662 KB
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• 作者单位：Ferdinand Ruedinger (1)
  Antonina Lavrentieva (1)
  Cornelia Blume (1)
  Iliyana Pepelanova (1)
  Thomas Scheper (1)
  
  1. Institute of Technical Chemistry, Gottfried Wilhelm Leibniz University Hanover, Callinstra脽e 5, 30167, Hanover, Germany
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Microbiology
  Microbial Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0614

文摘

Hydrogels have become one of the most popular platforms for three-dimensional (3D) cultivation of mammalian cells. The enormous versatility of hydrogel materials makes it possible to design scaffolds with predefined mechanical properties, as well as with desired biofunctionality. 3D hydrogel constructs have been used for a variety of applications, including tissue engineering of microorgan systems, drug delivery, cytotoxicity testing, and drug screening. Moreover, 3D culture is applied for investigating cellular physiology, stem cell differentiation, and tumor models and for studying interaction mechanisms between the extracellular matrix and cells. In this paper, we review current examples of performance-based hydrogel design for 3D cell culture applications. A major emphasis is placed on a description of how standard analytical protocols and imaging techniques are being adapted to analysis of 3D cell culture in hydrogel systems.