A three-dimensional microfluidic tumor cell migration assay to screen the effect of anti-migratory drugs and interstitial flow
详细信息 查看全文
- 作者：Johann Kalchman (1) (3)
Shingo Fujioka (2)
Seok Chung (4)
Yamato Kikkawa (5)
Toshihiro Mitaka (6)
Roger D. Kamm (7)
Kazuo Tanishita (1) (2)
Ryo Sudo (1) (2) - 关键词：3D cell migration ; Microfluidics ; Anti ; migratory drugs ; Interstitial flow
- 刊名：Microfluidics and Nanofluidics
- 出版年：2013
- 出版时间：June 2013
- 年：2013
- 卷：14
- 期：6
- 页码：969-981
- 全文大小：1028KB
- 参考文献：1. Ackerstaff E, Artemov D, Gillies RJ, Bhujwalla ZM (2007) Hypoxia and the presence of human vascular endothelial cells affect prostate cancer cell invasion and metabolism. Neoplasia 9:1138鈥?151 CrossRef
2. Chan DA, Giaccia AJ (2007) Hypoxia, gene expression, and metastasis. Cancer Metastasis Rev 26:333鈥?39 CrossRef
3. Curti BD, Urba WJ, Alvord G, Janik JE, Smith JW 2nd, Madara K, Longo DL (1993) Interstitial pressure of subcutaneous nodules in melanoma and lymphoma patients: changes during treatment. Cancer Res 53:2204鈥?207
4. Decaestecker C, Debeir O, Van Ham P, Kiss K (2007) Can anti-migratory drugs be screened in vitro? A review of 2D and 3D assays for the quantitative analysis of cell migration. Med Res Rev 27:149鈥?76 CrossRef
5. Fleury ME, Boardman KC, Swartz MA (2006) Autologous morphogen gradients by subtle interstitial flow and matrix interactions. Biophys J 91:113鈥?21 CrossRef
6. Garcia AH, Vega MD, Boettiger D (1999) Modulation of cell proliferation and differentiation through substrate-dependent changes in fibronectin conformation. Mol Biol Cell 10:785鈥?98
7. Gurski LA, Petrelli NJ, Jia X, Farach-Carson MC (2010) 3D Matrices for anti-cancer drug testing and development. Oncol Issues 25:20鈥?5
8. Gutmann R, Leunig M, Feyh J, Goetz AE, Messmer K, Kastenbauer E, Jain RK (1992) Interstitial hypertension in head and neck tumors in patients: correlation with tumor size. Cancer Res 52:1993鈥?995
9. Haessler U, Teo JCM, Foretay D, Renaud P, Swartz MA (2011) Migration dynamics of breast cancer cells in a tunable 3D interstitial flow chamber. Integr Biol. doi:10.1039/C1IB00128K
10. Haga A, Funasaka T, Niinaka Y, Raz A, Nagase H (2003) Autocrine motility factor signaling induces tumor apoptotic resistance by regulations Apaf-1 and Caspase-9 apoptosome expression. Int J Cancer 107:707鈥?14 CrossRef
11. Hanahan D, Weinberg RA (2011) Hallmarks of cancer: the next generation. Cell 144:646鈥?74 CrossRef
12. Hou J, Wang D, Zhang R, Wang H (2008) Experimental therapy of hepatoma with artemisinin and its derivatives: in vitro and in vivo activity, chemosensitization, and mechanisms of action. Clin Cancer Res 14:5519鈥?530 CrossRef
13. Jeanes A, Gottardi CJ, Yap AS (2008) Cadherins and cancer: how does cadherin dysfunction promote tumor progression? Oncogene 27:6920鈥?929 CrossRef
14. Kinoshita K, Taupin DR, Itoh H, Podolsky DK (2000) Distinct pathways of cell migration and antiapoptotic response to epithelial injury: structure-function analysis of human intestinal trefoil factor. Mol Cell Biol 20:4680鈥?690 CrossRef
15. Liotta LA (1986) Tumor invasion and metastase: role of the extracellular matrix. Cancer Res 46:1鈥? CrossRef
16. Polacheck WJ, Charest JL, Kamm RD (2011) Interstitial flow influences direction of tumor cell migration through competing mechanisms. Proc Natl Acad Sci USA 108:11115鈥?1120 CrossRef
17. Raju B, Haug SR, Ibrahim SO, Heyeraas KJ (2008) High interstitial fluid pressure in rat tongue cancer is related to increased lymph vessel area, tumor size, invasiveness and decreased body weight. J Oral Pathol Med 37:137鈥?44 CrossRef
18. Sekharam M, Zhao H, Sun M, Fang Q, Zhang Q, Yuan Z, Dan HC, Boulware D, Cheng JQ, Coppola D (2003) Insulin-like growth factor-1 receptor enhances invasion and induces resistance to apoptosis of colon cancer cells through the Akt/Bcl-XL pathway. Cancer Res 63:7708鈥?716
19. Shieh AC, Swartz MA (2011) Regulation of tumor invasion by interstitial fluid flow. Phys Biol 8:015012 CrossRef
20. Shields JD, Fleury ME, Yong C, Tomei AA, Randolph GJ, Swartz MA (2007) Autologous chemotaxis as a mechanism of tumor cell homing to lymphatics via interstitial flow and autocrine CCR7 signaling. Cancer Cell 11:526鈥?38 CrossRef
21. Shin Y, Han S, Jeon JS, Yamamoto K, Zervantonakis IK, Sudo R, Kamm RD, Chung S (2012) Microfluidic assay to analyze cellular morphogenesis that can incorporate hydrogels. Nat Protoc 7:1247鈥?259 CrossRef
22. Sudo R, Chung S, Zervantonakis IK, Vickerman V, Toshimitsu Y, Griffith LG, Kamm RD (2009) Transport-mediated angiogenesis in 3D epithelial coculture. FASEB J 23:2155鈥?164 CrossRef
23. Weifeng T, Feng S, Xiangji L, Changging S, Zhiquan Q, Huazhong Z, Peining Y, Yong Y, Mengchao W, Xiaoging J, Wan-Yee L (2011) Artemisinin inhibits in vitro and in vivo invasion and metastasis of human hepatocellular carcinoma cells. Phytomedicine 18:158鈥?62 CrossRef
24. Weigelt B, Lo AT, Park CC, Gray JW, Bissell MJ (2009) HER2 signaling pathway activation and response of breast cancer cells to HER2-targeting agents is dependent strongly on the 3D microenvironment. Breast Cancer Res Treat 122:35鈥?3 CrossRef
25. Xin Y, Li XL, Wang YP, Zhang SM, Zheng HC, Wu DY, Zhang YC (2001) Relationship between phenotypes of cell-function differentiation and pathobiological behavior of gastric carcinomas. World J Gastroenterol 7:53鈥?9
26. Yang Z, Rayala S, Nguyen D, Vladlamudi RK, Chen S, Kumar R (2005) Pak1 phosphorylation of snail, a master regulator of epithelial-to-mesenchyme transition, modulates snail鈥檚 subcellular localization and functions. Cancer Res 65:3179鈥?184
27. Zheng HC, Li YL, Sun JM, Yang XF, Li XH, Jiang WG, Zhang YC, Xin Y (2003) Growth, invasion, metastasis, differentiation, angiogenesis and apoptosis of gastric cancer regulated by expression of PTEN encoding products. World J Gastroenterol 9:1662鈥?666 - 作者单位：Johann Kalchman (1) (3)
Shingo Fujioka (2)
Seok Chung (4)
Yamato Kikkawa (5)
Toshihiro Mitaka (6)
Roger D. Kamm (7)
Kazuo Tanishita (1) (2)
Ryo Sudo (1) (2)
1. School of Integrated Design Engineering, Keio University, Yokohama, Japan
3. Ecole Centrale de Lille, Villeneuve-d鈥橝scq, France
2. Department of System Design Engineering, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, 223-8522, Japan
4. School of Mechanical Engineering, Korea University, Seoul, Korea
5. Laboratory of Clinical Biochemistry, Tokyo University of Pharmacy and Life Sciences, Tokyo, Japan
6. Department of Tissue Development and Regeneration, Research Institute for Frontier Medicine, Sapporo Medical University School of Medicine, Sapporo, Japan
7. Departments of Mechanical Engineering and Biological Engineering, Massachusetts Institute of Technology, Cambridge, USA - ISSN：1613-4990
文摘
Most anti-cancer drug screening assays are currently performed in two dimensions, on flat, rigid surfaces. However, there are increasing indications that three-dimensional (3D) platforms provide a more realistic setting to investigate accurate morphology, growth, and sensitivity of tumor cells to chemical factors. Moreover, interstitial flow plays a pivotal role in tumor growth. Here, we present a microfluidic 3D platform to investigate behaviors of tumor cells in flow conditions with anti-migratory compounds. Our results show that interstitial flow and its direction have significant impact on migration and growth of hepatocellular carcinoma cell lines such as HepG2 and HLE. In particular, HepG2/HLE cells tend to migrate against interstitial flow, and their growth increases in interstitial flow conditions regardless of the flow direction. Furthermore, this migratory activity of HepG2 cells is enhanced when they are co-cultured with human umbilical vein endothelial cells. We also found that migration activity of HepG2 cells attenuates under hypoxic conditions. In addition, the effect of Artemisinin, an anti-migratory compound, on HepG2 cells was quantitatively analyzed. The microfluidic 3D platform described here is useful to investigate more accurately the effect of anti-migratory drugs on tumor cells and the critical influence of interstitial flow than 2D culture models.