Effect of Drought and Nitrogen on Betulin and Oleanolic Acid Accumulation and OSC Gene Expression in White Birch Saplings

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• 作者：Jing Yin ; Tian Liang ; Siyao Wang ; Mengyan Zhang…
• 关键词：Birch (Betula platyphylla suk.) ; Betulin ; Oleanolic acid ; Gene expression ; Drought stress ; Nitrogen
• 刊名：Plant Molecular Biology Reporter
• 出版年：2015
• 出版时间：June 2015
• 年：2015
• 卷：33
• 期：3
• 页码：705-715
• 全文大小：1,453 KB
• 参考文献：Aguirre MC, Delporte C, Backhouse N, Erazo S, Letelier ME, Cassels BK, Silva X, Alegria S, Negrete RB (2006) Topical anti-inflammatory activity of 2 alpha-hydroxy pentacyclic triterpene acids from the leaves of Ugni molinae. Med Chem 14:5673-677View Article
  Basyunia M, Babaa S, Kinjoa Y, Oku H (2012) Salinity increases the triterpenoid content of a salt secretor and a non-salt secretor mangrove. Aquat Bot 97:17-3
  Chang S, Puryea J, Cairney J (1993) A simple and efficient method for isolating RNA from pine trees. Plant Mol Biol Report 11:693-99
  Elcan JM, Pezeshki SR (2002) Effects of flooding on susceptibility of Taxodium distichum L. seedlings to drought. Photosynthetica 40:177-82View Article
  Fǎlǎma? A, C?ntǎ P?nzaru S, Dehelean CA, Peev CI, Soica C (2011) Betulin and its natural resource as potential anticancer drug candidate seen by FT-Raman and FT-IR spectroscopy. Raman Spectrosc 42:97-07View Article
  Fritz C, Palacio-Rojas N, Feil R, Stitt M (2006) Regulation of secondary metabolism by the carbon nitrogen status in tobacco: nitrate inhibits large sectors of phenylpropanoid metabolism. Plant 46:533-48View Article
  Fujioka T, Kashiwada Y, Kilkuskie RE, Cosentino LM, Ballas LM, Jiang JB, Janzen WP, Chen IS, Lee KH (1994) Anti-AIDS agents.11. Betulinic acid and platanic acid as anti-HIV principles from Syzigium claviflorum, and the anti-HIV activity of structurally related triterpenoids. Nat Prod 57:243-47View Article
  Huang L, Lee KH, Chen CH (2006) Synthesis and anti-HIV activity of bi-functional BA derivatives. Bioorg Med Chem 14:2279-289View Article PubMed
  Jackson MB, Colmer TD (2005) Response and adaptation by plants to flooding stress. Ann Bot 96:501-05View Article PubMed Central PubMed
  Kashiwada Y, Sekiya M, Ikeshiro Y, Fujioka T, Kilgore NR, Wild CT, Allaway GP, Lee KH (2004) 3-O-Glutaryl-dihydrobetulin and related monoacyl derivatives as potent anti-HIV agents. Bioorg Med Chem Lett 14:5851-853
  Kashiwada Y, Sekiya M, Yamazaki K, Ikeshiro Y, Fujioka T, Yamagishi T, Kitagawa S, Takaishi Y (2007) Triterpenoids from the floral spikes of Betula platyphylla var. japonica and their reversing activity against multidrug-resistant cancer cells. J Nat Prod 70:623-27
  Li Q, Ren Q, Zhang YL (1994) Effects of ecological environment, collecting stages and storage time on chlorogenic Acid content of Lonicera ferdinandii Franch. China Chin Mat Med 18:594-95
  Liao JX, Wang GX (2003) The possible role of Glycyrrhizic acid in licorice adapted to desert habitats, in Chinese with English abstract. Plant Physiol Commun 39:367-70
  Liu Z (2000) Drought induced in vivo synthesis of camptothecin in Camptotheca acuminate seedlings. Physiol Plant 110:483-88
  Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2?ΔΔCT method. Methods 25:402-08View Article PubMed
  Maathuis FJM (2009) Physiological functions of mineral macronutrients. Curr Opin Plant Biol 12:250-25View Article PubMed
  Mukherjee R, Kumar V, Srivastava SK, Agarwal SK, Burman AC (2006) Betulinic acid derivatives as anticancer agents: structure activity relationship. Anticancer Agents Med Chem 6:271-79
  Nakagawa-Goto K, Yamada K, Taniguchi M, Tokuda H, Lee KH (2009) Cancer preventive agents 9 betulinic acid derivatives as potent cancer chemopreventive agents. Bioorg Med Chem Lett 19:3378-381
  Pezeshki SR, Santos MI (1998) Relationships among rhizosphere oxygen deficiency, root restriction, photosynthesis, and growth in baldcypress (Taxodium distichum L.) seedlings. Photosynthetica 35(3):381-90View Article
  Pisha EH, Chai IL, Chagwedera TE, Farnsworth NR, Cordell GA, Beecher CW, Fong HH, Kinghorn AD, Brown DM, Wani MC (1995) Discovery of betulinic acid as a selective inhibitor of human melanoma that functions by induction of apoptosis. Nat Med 1:1046-051View Article PubMed
  Rubio-Wilhelmi MM, Sanchez-Rodriguez E, Leyva R, Blasco B, Romero L, Blumwald E, Ruiz JM (2012a) Response of carbon and nitrogen-rich metabolites to nitrogen deficiency in PSARKTIPT tobacco plants. Plant Physiol Biochem 57:231-37
  Rubio-Wilhelmi MM, Sanchez-Rodriguez E, Rosales MA, Blasco B, Rios L, Romero Blumwald E (2012b) Ammonium formation and assimilation in PSARKTIPT tobacco transgenic plants under low N. Plant Physiol 169:157-62View Article
  Sanchez E, Rivero RM, Ruiz JM, Romero L (2004) Changes in biomass, enzymatic activity and protein concentration in roots and leaves of green bean plants (Phaseolus vulgaris L. cv. Strike) under high NH4NO3 application rates. Sci Hortic 99:237-48View Article
  Saxena BB, Zhu L, Hao M, Kisilis E, Katdare M, Oktem O, Bomshteyn A, Rathnam PB (2006) Boc-lysinate-betulonic acid: a potent, anti-prostate cancer agent. Med Chem 14:6249-258
  Scheible WR, Morcuende CRT, Frizt CD, Palacios-Rojas N, Schindelash D (2004) Genome-wide reprogramming of primary and secondary metabolism, protein synthesis, cellular growth p
• 作者单位：Jing Yin (1)
  Tian Liang (1)
  Siyao Wang (1)
  Mengyan Zhang (1)
  Jialei Xiao (3)
  Yaguang Zhan (1) (2)
  Chunxiao Li (1)
  
  1. College of Life Science, Northeast Forestry University, Harbin, 150040, China
  3. College of Life Science, Northeast Agriculture University, Harbin, 150010, China
  2. Key Laboratory of Forest Tree Genetic Improvement and Biotechnology of Nation, Northeast Forestry University, Harbin, 150040, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Plant Physiology
  
• 出版者：Springer Netherlands
• ISSN：1572-9818

文摘

Betulin and oleanolic acid are pentacyclic triterpene compounds with a broad range of biological and medicinal properties. Herein, we demonstrated that 2-year-old white birch saplings were capable of synthesizing betulin and oleanolic acid, and the accumulation of these compounds was significantly affected by seasonal and environmental factors. Indeed, the triterpenes accumulated mainly in the stem bark, with little in the root bark and leaves. From May to October, betulin and oleanolic acid stem bark accumulation displayed an inverted “V-curve, peaking in late July to late August (warmest summer months in Haerbin, China). Interestingly, low nitrogen levels promoted root bark and leaf accumulation of oleanolic acid, while high nitrogen amounts were more favorable to oleanolic acid and betulin accumulation in the stem bark. Moderate and mild drought during the accumulation peak period promoted oleanolic acid and betulin accumulation in the stem bark. Serious shortage of soil moisture inhibited the accumulation of both triterpenes. From July to August, high nitrogen (N3) treatment increased lupeol synthase (BPW) and beta-amyrin synthase (BPY) gene expression in the stem bark. Water stress, especially mild and moderate, induced BPW and BPY expression and downregulated cycloartenol synthase (BPX) in the stem bark. This study provides a strong basis for understanding triterpenoid synthesis in birch at the molecular level.