Analysis of three types of triterpenoids in tetraploid white birches (Betula platyphylla Suk.) and selection of plus trees

详细信息    查看全文

• 作者：Sui Wang ; Hui Zhao ; Jing Jiang ; Guifeng Liu ; Chuanping Yang
• 关键词：Betula platyphylla Suk ; Triterpenoids ; High performance liquid chromatography (HPLC) ; Tetraploid ; Diploid ; Factor analysis
• 刊名：Journal of Forestry Research
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：26
• 期：3
• 页码：623-633
• 全文大小：636 KB
• 参考文献：Alakurtti S, M?kel? T, Koskimies S, Yli-Kauhaluoma J (2006) Pharmacological properties of the ubiquitous natural product betulin. Eur J Pharm Sci 29(1):1-3PubMed View Article
  Caruso I, Lepore L, De Tommasi N, Dal Piaz F, Frusciante L, Aversano R, Garramone R, Carputo D (2011) Secondary metabolite profile in induced tetraploids of wild Solanum commersonii Dun. Chem Biodivers 8(12):2226-237PubMed View Article
  Chen Q, Liu J, Zhang H, He G, Fu M (2009) The betulinic acid production from betulin through biotransformation by fungi. Enzyme Microb Tech 45(3):175-80View Article
  Claude B, Morin P, Lafosse M, Andre P (2004) Evaluation of apparent formation constants of pentacyclic triterpene acids complexes with derivatized β- and γ-cyclodextrins by reversed phase liquid chromatography. J Chromatogr A 1049(1-):37-2PubMed View Article
  De Jesus-Gonzalez L, Weathers PJ (2003) Tetraploid Artemisia annua hairy roots produce more artemisinin than diploids. Plant Cell Rep 21(8):809-13PubMed
  Dhawan OP, Lavania UC (1996) Enhancing the productivity of secondary metabolites via induced polyploidy: a review. Euphytica 87(2):81-9View Article
  Dolan JW (2002) Peak tailing and resolution. LCGC North Am 20(5):430-36
  Fu JY, Qian LB, Zhu LG, Liang HT, Tan YN, Lu HT, Lu JF, Wang HP, Xia Q (2011) Betulinic acid ameliorates endothelium-dependent relaxation in L-NAME-induced hypertensive rats by reducing oxidative stress. Eur J Pharm Sci 44(3):385-91PubMed View Article
  Fujioka T, Kashiwada Y, Kilkuskie RE, Cosentino LM, Ballas LM, Jiang JB, Janzen WP, Chen IS, Lee KH (1994) Anti-AIDS agents, 11. Betulinic acid and platanic acid as anti-HIV principles from Syzigium claviflorum, and the anti-HIV activity of structurally related triterpenoids. J Nat Prod 57(2):243-47PubMed View Article
  Guo S, Duan J, Tang Y, Su S, Shang E, Ni S, Qian D (2009) High-performance liquid chromatography—two wavelength detection of triterpenoid acids from the fruits of Ziziphus jujuba containing various cultivars in different regions and classification using chemometric analysis. J Pharmaceut Biomed 49(5):1296-302View Article
  Huang L, Li J, Ye H, Li C, Wang H, Liu B, Zhang Y (2012) Molecular characterization of the pentacyclic triterpenoid biosynthetic pathway in Catharanthus roseus. Planta 236(5):1571-581PubMed View Article
  Kim Y, Hahn E, Murthy HN, Paek K (2004) Effect of polyploidy induction on biomass and ginsenoside accumulations in adventitious roots of ginseng. Journal of Plant Biology 47(4):356-60View Article
  Krasutsky PA (2006) Birch bark research and development. Nat Prod Rep 23(6):919-42PubMed View Article
  Kromidas S, Kuss HJ (2009) Quantification in LC and GC: a practical guide to good chromatographic data. Wiley-VCH, Weinheim, p 8
  Lavania UC, Srivastava S, Lavania S, Basu S, Misra NK, Mukai Y (2012) Autopolyploidy differentially influences body size in plants, but facilitates enhanced accumulation of secondary metabolites, causing increased cytosine methylation. Plant J 71(4):539-49PubMed View Article
  Liu J (1995) Pharmacology of oleanolic acid and ursolic acid. J Ethnopharmacol 49(2):57-8PubMed View Article
  Madlung A (2013) Polyploidy and its effect on evolutionary success: old questions revisited with new tools. Heredity (Edinb) 110(2):99-04View Article
  MobileReference (ed) (2008) The illustrated encyclopedia of trees and shrubs: an essential guide to trees and shrubs of the world. Boston, MobileReference
  Ng DW, Zhang C, Miller M, Palmer G, Whiteley M, Tholl D, Chen ZJ (2011) cis- and trans-Regulation of miR163 and target genes confers natural variation of secondary metabolites in two Arabidopsis species and their allopolyploids. Plant Cell 23(5):1729-740PubMed Central PubMed View Article
  Pai SR, Nimbalkar MS, Pawar NV, Dixit GB (2011) Optimization of extraction techniques and quantification of betulinic acid (BA) by RP-HPLC method from Ancistrocladus heyneanus Wall. Ex Grah. Ind Crop Prod 34(3):1458-464View Article
  Pato?ka J (2003) Biologically active pentacyclic triterpenes and their current medicine signification. J Appl Biomed 1:7-2
  Pisha E, Chai H, Lee IS, Chagwedera TE, Farnsworth NR, Cordell GA, Beecher CW, Fong HH, Kinghorn AD, Brown DM, Et A (1995) Discovery of betulinic acid as a selective inhibitor of human melanoma that functions by induction of apoptosis. Nat Med 1(10):1046-051PubMed View Article
  Pollier J, Goossens A (2012) Oleanolic acid. Phytochemistry 77:10-5PubMed View Article
  Ranney TG (2006) Polyploidy: from evolution to new plant development. Combined proceedings international plant propagators-society, vol 56, pp 137-42
  Salin O, Alakurtti S, Pohjala L, Siiskonen A, Maass V, Maass M, Yli-Kauhaluoma J, Vuorela P (2010) Inhibitory effect of the natural product betulin and its derivatives against the intracellular bacterium Chlamydia pneumoniae. Biochem Pharmacol 80(8):1141-151PubMed View Article
  Schinor EC, Salvado
• 作者单位：Sui Wang (1)
  Hui Zhao (1)
  Jing Jiang (1)
  Guifeng Liu (1)
  Chuanping Yang (1)
  
  1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040, China
  
• 刊物主题：Forestry;
• 出版者：Springer Berlin Heidelberg
• ISSN：1993-0607

文摘

Betulin, oleanolic acid, and betulinic acid are naturally occurring pentacyclic triterpenoids that have significant medicinal value. Considerable amounts of these triterpenoids are available in the outer bark of white birch. In this study, we used ultrasound-assisted extraction (UAE) to extract triterpenoids from birch bark rapidly and with high efficiency. Using high performance liquid chromatography (HPLC), three types of triterpenoids were separated and detected. We examined the differences among triterpenoids extracted from diploid versus tetraploid white birch. Then, we used factor analysis to screen out tetraploid white birches with comprehensively excellent performance. The results indicate that the optimum conditions for extraction include the use of ethanol as an extraction solvent, a solid-to-liquid ratio of 0.1?g/10?ml, ultrasonic power set at 100?W, a temperature of 60?°C and an extraction time of 15?min. A reversed-phase C18 column (4.6?mm?×?250?mm?×?5?μm) with a column temperature of 30?°C and the mobile phase composed of A (acetonitrile) and B (0.1?% aqueous phosphoric acid, v/v) at a flow rate of 0.5?ml/min were used, and the detection wavelength was 195?nm. No significant difference was observed between diploid and tetraploid white birch in terms of the content of three types of triterpenoids (at a confidence level of 0.05). As triterpenoid content, height, and DBH (diameter at breast height) are strongly interrelated, we used factor analysis to evaluate all individuals, and we screened out six plus trees with excellent comprehensive characters.