BpMADS12 gene role in lignin biosynthesis of Betula platyphylla Suk by transcriptome analysis

详细信息    查看全文

• 作者：Huiyu Li ; Yang Yang ; Zijia Wang ; Xiaohong Guo ; Feifei Liu…
• 刊名：Journal of Forestry Research
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：27
• 期：5
• 页码：1111-1120
• 全文大小：953 KB
• 刊物主题：Forestry;
• 出版者：Springer Berlin Heidelberg
• ISSN：1993-0607
• 卷排序：27

文摘

MADS-box transcription factors show highly diverse regulatory functions in a wide variety of organisms. In this study, we characterized a MADS-box gene (BpMADS12) from the white birch (Betula platyphylla Suk). This gene is a member of the suppressor of overexpression of CO 1/tomato MADS 3 class of MADS-box genes. We generated lines overexpressing BpMADS12 and found that these had higher levels of lignin compared to that observed in nontransgenic lines. Transcriptome analysis revealed numerous changes in gene expression patterns. In total, 8794 differentially expressed genes were identified, including 5006 upregulated unigenes and 3788 downregulated unigenes in BpMADS-overexpression lines. Differentially expressed genes involved in the pathways for lignin and brassinosteroid biosynthesis were significantly enriched and may have contributed to phenotypic changes. The results from a quantitative RT-PCR analysis were consistent those obtained with the transcriptome analysis. Our transcriptome analysis, in combination with measurement of lignin level, indicated that BpMADS12 promotes lignin synthesis through regulation of key enzymes in response to brassinosteroid signaling. These results suggest that this MADS-box protein is crucial to all subsequent structural events and provide a good foundation for studies aiming to elucidate the developmental mechanisms underlying formation of wood.KeywordsBetula platyphyllaBpMADS12Lignin biosynthesisTranscriptome analysis