Gene overexpression and gene silencing in Birch using an Agrobacterium-mediated transient expression system

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• 作者：Yan Zhang (1)
  Yucheng Wang (1)
  Chao Wang (1) wangchao7993@yahoo.com.cn
• 关键词：Betula platyphylla Suk – ; Agrobacterium – ; Transient gene expression – ; Silencing – ; CCR
• 刊名：Molecular Biology Reports
• 出版年：2012
• 出版时间：May 2012
• 年：2012
• 卷：39
• 期：5
• 页码：5537-5541
• 全文大小：287.9 KB
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• 作者单位：1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, Harbin, 150040 People鈥檚 Republic of China
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Animal Anatomy, Morphology and Histology
  Animal Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-4978

文摘

As transient expression systems are effective methods for the functional characterization of genes, a transient gene expression and silencing system was developed for Betula platyphylla Suk (Chinese Birch). Firstly, the cinnamoyl-CoA reductase (CCR) gene and its promoter were isolated from Chinese Birch. The vectors for overexpression of CCR and RNAi-based silence of CCR were constructed and transformed into Agrobacterium, respectively. Overexpression and silence of the CCR gene were respectively, performed on Birch seedlings using an Agrobacterium-mediated transient expression system. The expression levels of CCR were determined using real-time PCR. The results showed that the transcripts of CCR notably increased in the Birch plants transformed with the CCR overexpression construct, and notably decreased in plants transformed with the silencing construct when compared with nontransgenic plants. These studies confirmed that this transient genetic transformation system works well on Birch plants, and can be used for the functional characterization of genes and protein production in Birch.