Optimizing mesoderm progenitor selection and three-dimensional microniche culture allows highly efficient endothelial differentiation and ischemic tissue repair from human pluripotent stem cells
详细信息 查看全文
- 作者：Fengzhi Zhang ; Lin Wang ; Yaqian Li ; Wei Liu ; Fuyu Duan…
- 关键词：Human pluripotent stem cells ; Endothelial cells ; MESP1 ; 3D culture ; Vascularization
- 刊名：Stem Cell Research & Therapy
- 出版年：2017
- 出版时间：December 2017
- 年：2017
- 卷：8
- 期：1
- 全文大小：3240KB
- 刊物主题：Stem Cells; Cell Biology;
- 出版者：BioMed Central
- ISSN：1757-6512
- 卷排序：8
文摘
BackgroundGeneration of large quantities of endothelial cells is highly desirable for vascular research, for the treatment of ischemia diseases, and for tissue regeneration. To achieve this goal, we developed a simple, chemically defined culture system to efficiently and rapidly differentiate endothelial cells from human pluripotent stem cells by going through an MESP1 mesoderm progenitor stage.