Characterization of endoplasmic streaming in Physarum polycephalum using direction sensitive laser Doppler microscopy

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• 作者：Tatiana I. Avsievich ; Sergey V. Frolov…
• 关键词：Sign ; sensitive sensitive laser Doppler microscopy ; Short ; time Fourier transform ; Plasmodium of Physarum polycephalum ; Non ; muscle motility ; Cellular respiration inhibitors
• 刊名：Optical and Quantum Electronics
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：48
• 期：2
• 全文大小：1,928 KB
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• 作者单位：Tatiana I. Avsievich (1)
  Sergey V. Frolov (1)
  Sergey G. Proskurin (1)
  
  1. Biomedical Engineering, Tambov State Technical University, 112 Michurinskaya, Tambov, Russia, 392032
  
• 刊物主题：Optics, Optoelectronics, Plasmonics and Optical Devices; Electrical Engineering; Characterization and Evaluation of Materials; Computer Communication Networks;
• 出版者：Springer US
• ISSN：1572-817X

文摘

Shuttle endoplasmic streaming in an isolated strand of Physarum polycephalum plasmodium under normal conditions and after treatment with cellular respiration inhibitors (KCN and SHAM) was investigated using sign-sensitive sensitive laser Doppler velocimetry. Spectral analysis (short-time Fourier transform) applied to the experimental velocity time dependencies V(t) of endoplasmic motility, reveals two distinct frequencies, ν₁ and ν₂, corresponding to two harmonic contributions in each V(t), meaning the presence of at list two internal oscillators. Treatment with the inhibitors causes full stop of the endoplasmic streaming with simultaneous suppression of both harmonic oscillators. After removal of the inhibitors system returns to its original state (as before treatment)—the activity of both peaks is gradually restored during 800 s. Despite the frequency values are different, the ratio, ν₂/ν₁, remains constant for all investigated cases and approximately is equal to two.