Encapsulation of silica nano-spheres with polymerized ionic liquid for selective isolation of acidic proteins
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- 作者：Lu Han ; Yang Shu ; Xiaofeng Wang ; Xuwei Chen…
- 关键词：Polymeric ionic liquid ; Silica nano ; sphere ; Acidic protein ; Isolation
- 刊名：Analytical and Bioanalytical Chemistry
- 出版年：2013
- 出版时间：November 2013
- 年：2013
- 卷：405
- 期：27
- 页码：8799-8806
- 全文大小：434KB
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Yang Shu (2)
Xiaofeng Wang (1)
Xuwei Chen (1)
Jianhua Wang (1) (3)
1. Research Center for Analytical Sciences, College of Sciences, Northeastern University, Box 332, Shenyang, 110189, China
2. Institute of Biological Technology, College of Sciences, Northeastern University, Shenyang, 110189, China
3. Synergetic Innovation Center of Chemical Science and Engineering, Tianjin, 300071, China - ISSN：1618-2650
文摘
A nanocomposite is prepared by encapsulating silica nano-spheres with polymerized ionic liquid in aqueous medium without use of any organic solvents. Vinyl groups are covalently introduced on to the surface of silica nano-spheres, which are then encapsulated by copolymerization of 1-vinyl-3-ethylimidazolium bromide (monomer) and 1,4-butanediyl-3,3-bis-l-vinylimidazolium dibromide (cross-linker) at room temperature. The derived nanocomposite, PIL@SiO2, provides a green adsorbent for protein sorption. PIL@SiO2 is selective toward acidic proteins, and its selectivity can be controlled via varying the amount of monomer used in the copolymerization process. At pH?6.0, use of 5?mg PIL@SiO2 nanocomposite results in a sorption efficiency of up to 95?% for 200?mg?L? ovalbumin in 1?mL sample solution. Electrostatic and hydrophobic interactions between PIL@SiO2 and protein species dominate the adsorption process. The ovalbumin adsorption behavior is consistent with the Langmuir model, giving a sorption capacity of 333.3?mg?g?. The retained ovalbumin is recovered by elution with 0.2?% SDS solution. Circular dichroism spectra reveal virtually no change to the α-helix content of ovalbumin after elimination of SDS by use of dialysis. In summary, high-purity ovalbumin is isolated from chicken egg-white by use of the PIL@SiO2 nanocomposite as adsorbent.