Astragalus Polysaccharide Inhibits Autophagy and Apoptosis from Peroxide-Induced Injury in C2C12 Myoblasts

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• 作者：Yi Yin ; Lu Lu ; Dongtao Wang ; Ying Shi ; Ming Wang…
• 关键词：Astragalus polysaccharides ; C2C12 ; Autophagy ; Apoptosis ; mTOR ; Independent ; Caspase ; 3
• 刊名：Cell Biochemistry and Biophysics
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：73
• 期：2
• 页码：433-439
• 全文大小：1,486 KB
• 参考文献：1.Lecker, S. H., Goldberg, A. L., & Mitch, W. E. (2006). Protein degradation by the ubiquitin-proteasome pathway in normal and disease states. Journal of the American Society of Nephrology, 17, 1807鈥?819.CrossRef PubMed
  2.Sandri, M. (2008). Signaling in muscle atrophy and hypertrophy. Physiology (Bethesda), 23, 160鈥?70.CrossRef
  3.Glass, D. J. (2003). Molecular mechanisms modulating muscle mass. Trends in Molecular Medicine, 9(8), 344鈥?50.CrossRef PubMed
  4.Cai, D., Frantz, J. D., Tawa, N. E, Jr, Melendez, P. A., Oh, B. C., Lidov, H. G., et al. (2004). IKKbeta/NF-kappaB activation causes severe muscle wasting in mice. Cell, 119(2), 285鈥?98.CrossRef PubMed
  5.Welle, S. L. (2009). Myostatin and muscle fiber size. Focus on 鈥淪mad2 and 3 transcription factors control muscle mass in adulthood鈥?and 鈥淢yostatin reduces Akt/TORC1/p70S6K signaling, inhibiting myoblast differentiation and myotube size鈥? American Journal of Physiology. Cell Physiology, 296(6), C1245鈥揅1247.CrossRef PubMed
  6.Sachdev, S., & Davies, K. J. (2008). Production, detection, and adaptive responses to free radicals in exercise. Free Radical Biology and Medicine, 44(2), 215鈥?23.CrossRef PubMed
  7.Kerkweg, U., Petrat, F., Korth, H. G., & de Groot, H. (2007). Disruption of skeletal myocytes initiates superoxide release: contribution of NADPH oxidase. Shock, 27(5), 552鈥?58.CrossRef PubMed
  8.Sim, M. K., Wong, Y. C., Xu, X. G., & Loke, W. K. (2014). Des-aspartate-angiotensin I attenuates ICAM-1 formation in hydrogen peroxide-treated C2C12 skeletal muscle cells and soleus muscle of mice subjected to eccentric exercise. Regulatory Peptides, 188, 40鈥?5.CrossRef PubMed
  9.del Rio, L. A., Sandalio, L. M., Corpas, F. J., Palma, J. M., & Barroso, J. B. (2006). Reactive oxygen species and reactive nitrogen species in peroxisomes. Production, scavenging, and role in cell signaling. Plant Physiology, 141(2), 330鈥?35.PubMed Central CrossRef PubMed
  10.Cheng, X. D., Jia, X. B., Feng, L., & Jiang, J. (2013). Study thought of material basis of secondary development of major traditional Chinese medicine varieties on basis of combination of in vivo and in vitro experiments. Zhongguo Zhong yao za zhi聽=聽Zhongguo zhongyao zazhi聽=聽China Journal of Chinese Materia Medica, 38(23), 4174鈥?180.PubMed
  11.Liu, Y. Q., Xin, T. R., Lu, X. Y., Ji, Q., Jin, Y., & Yang, H. D. (2007). Memory performance of hypercholesterolemic mice in response to treatment with soy isoflavones. Neuroscience Research, 57(4), 544鈥?49.CrossRef PubMed
  12.Zhang, C. L., Ren, H. J., Liu, M. M., Li, X. G., de Sun, L., Li, N., & Ming, L. (2014). Modulation of intestinal epithelial cell proliferation, migration, and differentiation in vitro by Astragalus polysaccharides. PLoS ONE, 9(8), e106674.PubMed Central CrossRef PubMed
  13.Chen, H. L., Li, D. F., Chang, B. Y., Gong, L. M., Dai, J. G., & Yi, G. F. (2003). Effects of Chinese herbal polysaccharides on the immunity and growth performance of young broilers. Poultry Science, 82(3), 364鈥?70.CrossRef PubMed
  14.Dang, S. S., Jia, X. L., Song, P., Cheng, Y. A., Zhang, X., Sun, M. Z., & Liu, E. Q. (2009). Inhibitory effect of emodin and Astragalus polysaccharide on the replication of HBV. World Journal of Gastroenterology : WJG, 15(45), 5669鈥?673.PubMed Central CrossRef PubMed
  15.Guo, F. C., Kwakkel, R. P., Williams, C. B., Suo, X., Li, W. K., & Verstegen, M. W. (2005). Coccidiosis immunization: effects of mushroom and herb polysaccharides on immune responses of chickens infected with Eimeria tenella. Avian Diseases, 49(1), 70鈥?3.CrossRef PubMed
  16.Guo, F. C., Williams, B. A., Kwakkel, R. P., Li, H. S., Li, X. P., Luo, J. Y., et al. (2004). Effects of mushroom and herb polysaccharides, as alternatives for an antibiotic, on the cecal microbial ecosystem in broiler chickens. Poultry Science, 83(2), 175鈥?82.CrossRef PubMed
  17.Tin, M. M., Cho, C. H., Chan, K., James, A. E., & Ko, J. K. (2007). Astragalus saponins induce growth inhibition and apoptosis in human colon cancer cells and tumor xenograft. Carcinogenesis, 28(6), 1347鈥?355.CrossRef PubMed
  18.Guo, L., Bai, S. P., Zhao, L., & Wang, X. H. (2012). Astragalus polysaccharide injection integrated with vinorelbine and cisplatin for patients with advanced non-small cell lung cancer: effects on quality of life and survival. Medical Oncology, 29(3), 1656鈥?662.CrossRef PubMed
  19.Liu, X. N., Zhang, C. Y., Jin, X. D., Li, Y. Z., Zheng, X. Z., & Li, L. (2007). Inhibitory effect of schisandrin B on gastric cancer cells in vitro. World Journal of Gastroenterology : WJG, 13(48), 6506鈥?511.CrossRef PubMed
  20.Zhao, P., Su, G., Xiao, X., Hao, E., Zhu, X., & Ren, J. (2008). Chinese medicinal herb Radix Astragali suppresses cardiac contractile dysfunction and inflammation in a rat model of autoimmune myocarditis. Toxicology Letters, 182(1鈥?), 29鈥?5.CrossRef PubMed
  21.Liu, M., Wu, K., Mao, X., Wu, Y., & Ouyang, J. (2010). Astragalus polysaccharide improves insulin sensitivity in KKAy mice: regulation of PKB/GLUT4 signaling in skeletal muscle. Journal of Ethnopharmacology, 127(1), 32鈥?7.CrossRef PubMed
  22.Lu, L., Wang, D. T., Shi, Y., Yin, Y., Wei, L. B., Zou, Y. C., et al. (2013). Astragalus polysaccharide improves muscle atrophy from dexamethasone- and peroxide-induced injury in vitro. International Journal of Biological Macromolecules, 61, 7鈥?6.CrossRef PubMed
  23.Ku, B. M., Lee, Y. K., Jeong, J. Y., Ryu, J., Choi, J., Kim, J. S., et al. (2011). Caffeine inhibits cell proliferation and regulates PKA/GSK3beta pathways in U87MG human glioma cells. Molecules and Cells, 31(3), 275鈥?79.PubMed Central CrossRef PubMed
  24.Kamsteeg, M., Rutherford, T., Sapi, E., Hanczaruk, B., Shahabi, S., Flick, M., et al. (2003). Phenoxodiol鈥揳n isoflavone analog鈥搃nduces apoptosis in chemoresistant ovarian cancer cells. Oncogene, 22(17), 2611鈥?620.CrossRef PubMed
  25.Kruger, N. J. (1994). The Bradford method for protein quantitation. Methods in Molecular Biology, 32, 9鈥?5.PubMed
  26.Bongers, K. S., Fox, D. K., Kunkel, S. D., Stebounova, L. V., Murry, D. J., Pufall, M. A. et al. (2014). Spermine oxidase maintains basal skeletal muscle gene expression and fiber size, and is strongly repressed by conditions that cause skeletal muscle atrophy. American Journal of Physiology Endocrinology and Metabolism, ajpendo 00472 02014.
  27.Bodine, S. C. (2013). Disuse-induced muscle wasting. The International Journal of Biochemistry & Cell Biology, 45(10), 2200鈥?208.CrossRef
  28.Fry, C. S., & Rasmussen, B. B. (2011). Skeletal muscle protein balance and metabolism in the elderly. Current Aging Science, 4(3), 260鈥?68.CrossRef PubMed
  29.Li, F., Li, Y., Tang, Y., Lin, B., Kong, X., Oladele, O. A., & Yin, Y. (2014). Protective effect of myokine IL-15 against H2O 2-mediated oxidative stress in skeletal muscle cells. Molecular Biology Reports, 41(11), 7715鈥?722.CrossRef PubMed
  30.Yang, M., Lin, H. B., Gong, S., Chen, P. Y., Geng, L. L., Zeng, Y. M., & Li, D. Y. (2014). Effect of Astragalus polysaccharides on expression of TNF-alpha, IL-1beta and NFATc4 in a rat model of experimental colitis. Cytokine.
  31.Yang, Z., & Klionsky, D. J. (2009). An overview of the molecular mechanism of autophagy. Current Topics in Microbiology and Immunology, 335, 1鈥?2.PubMed Central PubMed
  32.Mammucari, C., Schiaffino, S., & Sandri, M. (2008). Downstream of Akt: FoxO3 and mTOR in the regulation of autophagy in skeletal muscle. Autophagy, 4, 524鈥?26.CrossRef PubMed
  33.Wang, W., Wen, Q., Xu, L., Xie, G., Li, J., Luo, J., et al. (2014). Activation of Akt/mTOR pathway is associated with poor prognosis of nasopharyngeal carcinoma. PLoS ONE, 9(8), e106098.PubMed Central CrossRef PubMed
  34.Wang, W. K., Lin, S. T., Chang, W. W., Liu, L. W., Li, T. Y., Kuo, C. Y., Hsieh, J. L., & Lee, C. H. (2014) Hinokitiol induces autophagy in murine breast and colorectal cancer cells. Environmental Toxicology.
  35.Li, G. Y., Xie, P., Li, H. Y., Hao, L., Xiong, Q., & Qiu, T. (2011). Involment of p53, Bax, and Bcl-2 pathway in microcystins-induced apoptosis in rat testis. Environmental Toxicology, 26(2), 111鈥?17.CrossRef PubMed
  36.Kilbride, S. M., & Prehn, J. H. (2013). Central roles of apoptotic proteins in mitochondrial function. Oncogene, 32(22), 2703鈥?711.CrossRef PubMed
  37.Chipuk, J. E., Fisher, J. C., Dillon, C. P., Kriwacki, R. W., Kuwana, T., & Green, D. R. (2008). Mechanism of apoptosis induction by inhibition of the anti-apoptotic BCL-2 proteins. Proceedings of the National Academy of Sciences of the United States of America, 105(51), 20327鈥?0332.PubMed Central CrossRef PubMed
  38.Ghate, N. B., Hazra, B., Sarkar, R., & Mandal, N. (2014). Heartwood extract of Acacia catechu induces apoptosis in human breast carcinoma by altering bax/bcl-2 ratio. Pharmacognosy Magazine, 10(37), 27鈥?3.PubMed Central CrossRef PubMed
  
• 作者单位：Yi Yin (1) (2) (3)
  Lu Lu (1) (2) (3)
  Dongtao Wang (4)
  Ying Shi (1)
  Ming Wang (1)
  Yanfeng Huang (1) (2) (3)
  Dexiu Chen (1) (2) (3)
  Cong Deng (5)
  Jiebin Chen (3)
  Peijia Lv (3)
  Yanjing Wang (1)
  Chengjie Li (3)
  Lian-Bo Wei (1) (2) (3)
  
  1. China School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, 510515, China
  2. Division of Nephrology, TCM-Integrated Hospital, Southern Medical University, Guangzhou, 510280, China
  3. Nephropathy Center of Integrated Traditional Chinese Medicine and Western Medicine, ZhuJiang Hospital, Southern Medical University, Guangzhou, 510280, China
  4. Department of Nephrology, Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Guangxi University of Chinese Medicine, Nanning, 530011, China
  5. Division of TCM, Guangzhou Nansha Central Hospital, Guangzhou First People鈥檚 Hospital, Guangzhou, 510180, China
  
• 刊物主题：Biochemistry, general; Pharmacology/Toxicology; Biotechnology; Cell Biology; Biophysics and Biological Physics;
• 出版者：Springer US
• ISSN：1559-0283

文摘

The aim is to study the effects and underlying mechanisms of astragalus polysaccharide (APS) on the peroxide-induced injury in C2C12 myoblasts in vitro. Cell viability in the presence or absence of APS was detected by the methyl thiazolyl tetrazolium colorimetric assay. The autophagosomes were observed by electron microscopy to examine the influence of APS on autophagy caused by H₂O₂ in C2C12 cells, and the percentage of apoptosis cells was measured by flow cytometry. To further confirm the effect of H₂O₂ on C2C12 cells, the protein expression of LC3 and RARP, which are the markers of autophagy and apoptosis, respectively, was analyzed by Western blot, as well as the expression levels of p-p70S6K, p70S6K, Bcl-2, Bax, cyto-C, and Caspase-3, to reveal the underlying mechanisms. We observed multiple effects of APS on C2C12 functionality. APS treatment of C2C12 cells at 1 mg/mL reduced cell viability to less than 70 %, and analysis by electron microscopy revealed that APS also reduced the number of H₂O₂-induced autophagosome formation. Similarly, APS abated the H₂O₂-mediated increase in cell apoptosis, which was accompanied by the inhibition of LC3 II and RARP that are normally upregulated by H₂O₂. The expression of p-p70S6K and p70S6K, however, remained unchanged in C2C12 cells in the Control, H₂O₂ and H₂O₂ + APS groups. In addition, APS promoted the expression of protein Bcl-2 in H₂O₂-treated C2C12 cells, but did not change Bax, thus reducing the Bax/Bcl-2 ratio that in turn prevented the release of cytochrome c and the activation of caspase-3. APS inhibits the autophagy and apoptosis induced by peroxide injury in C2C12 myoblasts through two independent signaling pathways: the mTOR-independent pathway for the inhibition of autophagy, and the caspase-3-dependent pathway for the suppression of apoptosis. Keywords Astragalus polysaccharides C2C12 Autophagy Apoptosis mTOR-Independent Caspase-3