Structural characterisation of neutrophil glycans by ultra sensitive mass spectrometric glycomics methodology
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- 作者：Ponnusamy Babu ; Simon J. North ; Jihye Jang-Lee ; Sara Chalabi ; Kathryn Mackerness ; Sean R. Stowell ; Richard D. Cummings ; Sara Rankin ; Anne Dell and Stuart M. Haslam
- 关键词：Mass spectrometry ; Neutrophil ; Glycomics ; Protein glycosylation
- 刊名：Glycoconjugate Journal
- 出版年：2009
- 出版时间：November, 2009
- 年：2009
- 卷：26
- 期：8
- 页码：975-986
- 全文大小：769.9 KB
文摘
Neutrophils are the most abundant white blood cells in humans and play a vital role in several aspects of the immune response. Numerous reports have implicated neutrophil glycosylation as an important factor in mediating these interactions. We report here the application of high sensitivity glycomics methodologies, including matrix assisted laser desorption ionisation (MALDI-TOF) and MALDI-TOF/TOF analyses, to the structural analysis of N- and O-linked carbohydrates released from two samples of neutrophils, prepared by two separate and geographically remote laboratories. The data produced demonstrates that the cells display a diverse range of sialylated and fucosylated complex glycans, with a high level of similarity between the two preparations.