Utilization of renewable agricultural residues for the production of extracellular halostable cellulase from newly isolated Halomonas sp. strain PS47

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• 作者：Pooja Shivanand (1)
  Gopal Mugeraya (1)
  Anubhav Kumar (1)
  
• 关键词：Halomonas sp. ; Moderately halophilic bacteria ; Halostable cellulase ; Agricultural residues ; Alkaline pretreatment ; Wheat bran
• 刊名：Annals of Microbiology
• 出版年：2013
• 出版时间：December 2013
• 年：2013
• 卷：63
• 期：4
• 页码：1257-1263
• 全文大小：
• 作者单位：Pooja Shivanand (1)
  Gopal Mugeraya (1)
  Anubhav Kumar (1)
  
  1. Department of Chemical Engineering, National Institute of Technology Karnataka, Surathkal, 575025, India
  
• ISSN：1869-2044

文摘

A newly isolated biopolymer-degrading halophilic bacterium, Halomonas sp. strain PS47, yielded higher cellulase activity (0.0076U/ml) in mineral salt medium (MM63). Activity increased to 0.029U/ml when carboxymethyl cellulose (0.5% w/v) was used as carbon source and further to 0.138U/ml when a combination of yeast extract and peptone was used as nitrogen source. Enzyme secretion was maximal during late exponential and stationary phases (0.15U/ml, 48h). Among different agro-residues (1% w/v), wheat bran gave the highest activity (0.12U/ml) at pH 7.5, 30 and 6% (w/v) NaCl. The cellulase exhibited higher activity at pH 7.1 and 50. The enzyme exhibited activity over a wide range of NaCl concentrations (0M). Optimum activity was at 0M NaCl. At 4M NaCl, activity was reduced to 65% of the initial value. The present investigation thus contributes to the limited information available on halostable cellulases.