Sodium butyrate-induced histone hyperacetylation up-regulating WT1 expression in porcine kidney fibroblasts

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• 作者：Wentao Tan ; Yue Chen ; Peipei An ; Aibing Wang ; Meiran Chu…
• 关键词：Histone acetylation ; Porcine kidney fibroblasts ; Sodium butyrate ; Swine ; Wilm’s tumor 1 gene
• 刊名：Biotechnology Letters
• 出版年：2015
• 出版时间：June 2015
• 年：2015
• 卷：37
• 期：6
• 页码：1195-1202
• 全文大小：3,143 KB
• 参考文献：An P, Yin Y, Fan A, Tan W, Gao F, Ma K, Tang B, Zhang X, Li Z (2013) The effects of the WT1 gene on apoptosis and development-related gene expression in porcine kidney fibroblasts and swine testis cells. Mol Reprod Dev 80:414-25View Article PubMed
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• 作者单位：Wentao Tan (1)
  Yue Chen (2)
  Peipei An (3)
  Aibing Wang (1)
  Meiran Chu (1)
  Liuxin Shi (1)
  Xiaoxiao Hou (1)
  Bo Tang (1)
  Xueming Zhang (1)
  Ziyi Li (1)
  
  1. Jilin Provincial Key Laboratory of Animal Embryo Engineering, The Center for Animal Embryo Engineering of Jilin Province, College of Veterinary Medicine, Jilin University, 5333 Xi-An Road, Changchun, 130062, People’s Republic of China
  2. School of Life Science and Technology, Harbin Institute of Technology, Harbin, 150001, People’s Republic of China
  3. State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences of CAAS, Chinese Academy of Agricultural Science, Changchun, 130112, People’s Republic of China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Biotechnology
  Applied Microbiology
  Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-6776

文摘

Objectives: Wilms-tumor 1 gene (WT1) is essential for the development of kidney and histone acetylation and is involved in its expression regulation in mice. However, whether WT1 expression is associated with histone acetylation in porcine kidney cells is unclear. Here, the effect of histone deacetylase inhibitor sodium butyrate (NaBu)-induced hyperacetylation on WT1 expression in porcine kidney fibroblasts (PKF) was examined. Results: Treatments of NaBu (1, 3, 6?mM) for 24?h increased PKF viability, and 24, 48?h-treatments of 1?mM NaBu enhanced PKF proliferation. WT1 mRNA levels were significantly elevated in NaBu-treated (1, 3?mM for 24, 48?h, respectively) PKF samples. Consistently, strengthened expression of WT1 protein and histone acetylation level were detected in NaBu-treated PKF cells. Conclusion: Together, NaBu-induced hyperacetylation up-regulates WT1 expression in PKF, suggesting the involvement of histone acetylation in the transcriptional modulation of WT1 in porcine kidney cells.