Hatchery decontamination of Sargassum muticum juveniles and adults using a combination of sodium hypochlorite and potassium iodide
详细信息 查看全文
- 作者：Philip D. Kerrison ; Hau Nhu Le ; Adam D. Hughes
- 关键词：Sargassum muticum ; Hypochlorite ; Iodide ; Protozoa ; Fluorometry ; Decontamination
- 刊名：Journal of Applied Phycology
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：28
- 期：2
- 页码：1169-1180
- 全文大小：660 KB
- 参考文献：Aguirre-Lipperheide M, Evans LV (1993) A sterilisation protocol for the Dictyotales (Phaeophyceae). J Phycol 29:243–251CrossRef
Amsler CD, Reed DC, Neushul M (1992) The microclimate inhabited by macroalgal propagules. Br Phycol J 27:253–270CrossRef
Andersen RA, Berges JA, Harrison PJ, Watanabe MM (2005) Appendix a—recipes for freshwater and seawater media. In: Andersen RA (ed) Algal culturing techniques. Elsevier Academic Press, London, pp 429–538
Anderson TW, Darling DA (1952) Asymptotic theory of certain “Goodness of Fit” criteria based on stochastic processes. Ann Math Stat 23:193–212CrossRef
Baweja P, Sahoo D, García-Jiménez RRR (2009) Seaweed tissue culture as applied to biotechnology: problems, achievements and prospects. Phycol Res 57:45–58CrossRef
Becker EW (1994) Microalgae: biotechnology and microbiology. Cambridge University Press, Cambridge
Chai Z, Huo Y, He Q, Huang X, Jiang X, He P (2014) Studies on breeding of Sargassum vachellianum on artificial reefs in Gouqi Island, China. Aquaculture 424–425:189–193
Cheang CC, Chu KH, Fujita D, Yoshida G, Hiraoka M, Critchley A, Choi HG, Duan D, Serisawa Y, Ang PO (2010) Low genetic variability of Sargassum muticum (Phaeophyceae) revealed by a global analysis of native and introduced populations. J Appl Phycol 46:1063–1074CrossRef
Cosgrove J, Borowitzka MA (2010) Chlorophyll fluorescence terminology: an introduction. In: Suggett DJ, Prášil O, Borowitzka MA (eds) Chlorophyll a fluorescence in aquatic sciences: methods and applications. Springer, Dordrecht, pp 1–17CrossRef
Coston-Clements L, Settle LR, Hoss DE, Cross FA (1991) Utilization of the Sargassum habitat by marine invertebrates and vertebrates - a review. NOAA Technical Memorandum NMFS-SEFSC-296: pp 32
Davison IR, Johnson LE, Brawley SH (1993) Sublethal stress in the intertidal zone: tidal emersion inhibits photosynthesis and retards development in embryos of the brown alga Pelvetia fastigiata. Oecologia 96:483–492CrossRef
Day JG, Thomas NJ, Achilles-Day UEM, Leakey RJG (2012) Early detection of protozoan grazers in algal biofuel cultures. Bioresour Technol 114:715–719CrossRef PubMed
Druehl LD, Hsiao SIC (1969) Axenic cultures of Laminariales in defined media. Phycologia 8:47–49CrossRef
Enriquez S, Borowitzka MA (2010) The use of the fluorescence signal in studies of seagrasses and macroalgae. In: Suggett DJ, Prášil O, Borowitzka MA (eds) Chlorophyll a fluorescence in aquatic sciences: methods and applications. Springer, Dordrecht, pp 187–208CrossRef
Fernandes DRP, Yokoya NS, Yoneshigue-Valentin Y (2011) Protocol for seaweed decontamination to isolate unialgal cultures. Rev Bras Farm 21:313–316CrossRef
Flameling I, Kromkamp J (1998) Light dependence of quantum yields for PSII charge separation and oxygen evolution in eucaryotic algae. Limnol Oceanogr 43:284–297CrossRef
Forrest BM, Hopkins GA, Dodgshun TJ, Gardner JPA (2007) Efficacy of acetic acid treatments in the management of marine biofouling. Aquaculture 262:319–332CrossRef
Fries L (1980) Axenic tissue cultures from the sporophytes of Laminaria digitata and Laminaria hyperborea (Phaeophyta). J Phycol 16:475–477CrossRef
Gall EA, Küpper FC, Kloareg B (2004) A survey of iodine content in Laminaria digitata. Bot Mar 47:30–37
Gibor A, Polne M, Biniamino M, Neushul M (1981) Exploratory studies of vegetative propagation of marine algae: procedure for obtaining axenic tissues. Proc Int Seaweed Symp 10:587–593
Grimm MR (1952) Iodine content of some marine algae. Pac Sci 6:318–323
Guillard RRL (1975) Culture of phytoplankton for feeding marine invertebrates. In: Smith WL, Chanley MH (eds) Culture of marine invertebrate animals. Plenum Press, New York, pp 26–60
Gusev MV, Tambiev AH, Kirikova NN, Shelyastina NN, Aslanyan RR (1987) Callus formation in seven species of agarophyte marine algae. Mar Biol 95:593–597CrossRef
Hong DD, Hien HM, Son PN (2007) Seaweeds from Vietnam used for functional food, medicine and biofertilizer. J Appl Phycol 19:817–826CrossRef
Hou X, Yan X (1998) Study on the concentration and seasonal variation of inorganic elements in 35 species of marine algae. Sci Total Environ 222:141–156CrossRef
Hsiao SIC, Druehl LD (1971) Environmental control of gametogenesis in Laminaria saccharina I the effects of light on culture media. Can J Bot 49:1503–1508CrossRef
Hwang EK, Ha DS, Baek JM, Wee MY, Park CS (2006a) Effects of pH and salinity on the cultivated brown alga Sargassum fulvellum and associated animals. Algae 21:317–321CrossRef
Hwang EK, Park CS, Baek JM (2006b) Artificial seed production and cultivation of the edible brown alga Sargassum fulvellum (Turner) C. Agardh: developing a new species for seaweed cultivation in Korea. J Appl Phycol 18:251–257
Kawachi M, Noël M-H (2005) Sterilisation and sterile technique. In: Andersen RA (ed) Algal culturing techniques. Elsevier Academic Press, London, pp 65–82
Kawashima Y, Tokuda H (1990) Callus formation in Ecklonia cava Kjellman (Laminariales, Phaeophyta). Hydrobiologia 204/205:375–380
Kerrison P, Le HN (2015) Environmental factors on egg liberation and germling production of Sargassum muticum. J Appl Phycol. doi:10.1007/s10811-015-0580-y
Kientz B, Thabard M, Cragg S, Pope J, Hellio C (2012) A new method for removing microflora from macroalgal surfaces: an important step for natural product discovery. Bot Mar 54:457–469
Kolber Z, Falkowski PG (1993) Use of active fluorescence to estimate phytoplankton photosynthesis in situ. Limnol Oceanogr 38:1646–1665CrossRef
Lawlor HJ, Borowitzka MA, McComb JA (1991) A rapid and inexpensive method for surface sterilisation of Ecklonia radiata (Phaeophyta) for tissue culture. Bot Mar 34:261–264CrossRef
Lesser MP (2006) Oxidative stress in marine environments: biochemistry and physiological ecology. Annu Rev Physiol 68:253–278CrossRef PubMed
Maxwell K, Johnson GN (2000) Chlorophyll fluorescence—a practical guide. J Exp Bot 51:659–668CrossRef PubMed
McCracken IR (1989) Purifying algal cultures—a review of chemical methods. Proc N S Inst Sci 38:145–168
Merrill JE, Gillingham DM (1991) Bull kelp cultivation handbook. National Coastal Resources Research and Development Institute, Portland, 60pp
Moreno-Garrido I, Canavate JP (2001) Assessing chemical compounds for controlling predator ciliates in outdoor mass cultures of the green algae Dunaliella salina. Aquac Eng 24:107–114CrossRef
Nishiyama Y, Murata N (2014) Revised scheme for the mechanism of photoinhibition and its application to enhance the abiotic stress tolerance of the photosynthetic machinery. Appl Microbiol Biotechnol 98:8777–8796CrossRef PubMed
Pang SJ, Chen LT, Zhuang DG, Fei XG, Sun JZ (2005) Cultivation of the brown alga Hizikia fusiformis (Harvey) Okamura: enhanced seedling production in tumbled culture. Aquaculture 245:321–329CrossRef
Pang SJ, Zhang ZH, Zhao HJ (2007) Cultivation of the brown alga Hizikia fusiformis (Harvey) Okamura: stress resistance of artificially raised young seedlings revealed by chlorophyll fluorescence measurement. J Appl Phycol 19:557–565CrossRef
Pang SJ, Liu F, Shan TF, Gao SQ, Zhang ZH (2009) Cultivation of the brown alga Sargassum horneri: sexual reproduction and seedling production in tank culture under reduced solar irradiance in ambient temperature. J Appl Phycol 21:413–422CrossRef
Post FJ, Borowitzka LJ, Borowitzka MA, Mackay B, Moulton T (1983) The protozoa of a Western Australian hypersaline lagoon. Hydrobiologia 105:95–113CrossRef
Ragni M, Airs RL, Hennige SJ, Suggett DJ, Warner ME, Geider RJ (2010) PSII photoinhibition and photorepair in Symbiodinium (Pyrrhophyta) differs between thermally tolerant and sensitive phylotypes. Mar Ecol Prog Ser 406:57–70CrossRef
Ramlov F, Plastino EM, Yokoya NS (2009) Efeitos do ágar no crescimento de explantes e na formação de calos em morfos pigmentares de Gracilaria domingensis (Kützing) Sonder ex Dickie (Gracilariales, Rhodophyta). Rev Bras Bot 32:607–615CrossRef
Reddy CRK, Jha B, Fujita Y, Ohno M (2008) Seaweed micropropagation techniques and their potentials: an overview. J Appl Phycol 20:609–617CrossRef
Redmond S, Kim JK, Yarish C, Pietrak M, Bricknell I (2014) Culture of Sargassum in Korea: techniques and potential for culture in the U.S. Marine Sea Grant College program, Orono, Maine. 13 pp
Reed DC, Neushul M, Ebeling AW (1991) Role of settlement density on gametophyte growth and reproduction in the kelps Pterygophora californica and Macrocystis pyrifera (Phaeophyceae). J Phycol 27:361–366CrossRef
Rød KK (2012) Sori disinfection in cultivation of Saccharina latissima. Norwegian University of Science and Technology, Trondheim
Sánchez-Saavedra MP, Voltolina D, Simental J, Carbajal-Miranda MJ (2008) Removal of epiphytes of the kelp Macrocystis pyrifera (L.) Agardh using different biocides. Hidrobiológica 18:99–104
Schreiber U, Schliwa U, Bilger W (1986) Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer. Photosynth Res 10:51–62CrossRef PubMed
Sherr EB, Sherr BF (2002) Significance of predation by protists in aquatic microbial food webs. Antonie Van Leeuwenhoek 81:293–308CrossRef PubMed
Sohn CH (1993) Porphyra, Undaria and Hizikia cultivation in Korea. Algae 8:207–216
Titlyanov EA, Titlyanova TV, Pham VH (2012) Stocks and the uses of economic marine macrophytes of Vietnam. Russ J Mar Biol 38:285–298CrossRef
Tsukidate J (1992) Ecology of Sargassum spp. and Sargassum forest formation. NOAA Tech Rep NMES 106:63–72
Wilson TA, Graham S, Miles SA, Knox A, Parker R, Macrae MT, Meynell AD, Meynell GG, Elinor W (1964) Topley and Wilson’s principles of bacteriology and immunity. Edward Arnold, London
Xie EY, Liu DC, Jia C, Chen X, Li YB (2013) Artificial seed production and cultivation of the edible brown alga Sargassum naozhouense Tseng et Lu. J Appl Phycol 25:513–522CrossRef
Yan ZM (1984) Studies on tissue culture of Laminaria japonica and Undaria pinnatifida. Hydrobiologia 115/116:314–316
Yoon JT, Sun SM, Chung G (2014) Sargassum bed restoration by transplantation of germlings grown under protective mesh cages. J Appl Phycol 26:505–509CrossRef
Zhao Z, Zhao F, Yao J, Lu J, Ang PO, Duan D (2008) Early development of germlings of Sargassum thunbergii (Fucales, Phaeophyta) under laboratory conditions. J Appl Phycol 20:475–481 - 作者单位：Philip D. Kerrison (1)
Hau Nhu Le (2)
Adam D. Hughes (1)
1. SAMS, Scottish Marine Institute, Dunbeg, Argyll, PA37 1QA, UK
2. Nha Trang Institute of Technology and Application (NITRA), Vietnam Academy of Science and Technology (VAST), 2 Hung Vuong Street, Nha Trang City, Vietnam - 刊物主题：Plant Sciences; Freshwater & Marine Ecology; Plant Physiology; Ecology;
- 出版者：Springer Netherlands
- ISSN：1573-5176
文摘
Phaeophyte macroalgae of the genus Sargassum, including Sargassum muticum, are under investigation as a cultivation crop. Overgrowth or grazing reduces the value and productivity of the crop. This can occur both in the hatchery and during growth at sea, although juvenile stages are especially vulnerable. A decontamination protocol could be used to prevent this. Following a screening procedure, sodium hypochlorite (NaClO) and potassium iodide (KI) were selected for further study. The lethal concentrations for protozoans associated with S. muticum zygotes were 1 % KI, 0.75 % NaClO or a combination of 0.5 % KI and 0.38 % NaClO. A 3-min exposure to these treatments was able to eliminate protozoa from branchlets 20–80 % of the time. NaClO caused large reductions in the operating efficiency of photosystem II (Fq ’/Fm ’), particularly in juveniles (74 ± 17 %), which had still not fully recovered after 17 days. This also halved juvenile density and caused negative growth. One percent KI reduced Fq ’/Fm ’ in adults by 29 ± 11 %, with recovery by day 10. In juveniles, growth was reduced by 65 % and Fq ’/Fm ’ by 61 ± 11 % with recovery after 17 days. A combined treatment of 0.5 % KI and 0.38 % NaClO had far milder effects: reducing adult and juveniles Fq ’/Fm ’ by 6 ± 3 and 34 ± 9 % respectively, with full recovery by day 10. A reduction in juvenile growth was also seen between day 0–10 (32 %); however, size was no different to the control by day 20. This combined treatment is therefore suitable for decontamination of both juveniles and adult S. muticum tissue.