Characterisation of P-glycoprotein-9.1 in Haemonchus contortus

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• 作者：Pablo Godoy ; Hua Che ; Robin N. Beech ; Roger K. Prichard
• 关键词：Macrocyclic lactone resistance ; P ; glycoprotein ; Transport studies ; Heterologous expression system ; Protein localisation ; Ivermectin ; Abamectin ; Moxidectin
• 刊名：Parasites & Vectors
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：9
• 期：1
• 全文大小：627 KB
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• 作者单位：Pablo Godoy (1)
  Hua Che (1)
  Robin N. Beech (1)
  Roger K. Prichard (1)
  
  1. Institute of Parasitology, Macdonald campus, McGill University, 21,111 Lakeshore Road, Sainte Anne-de-Bellevue, H9X3V9, QC, Canada
  
• 刊物主题：Parasitology; Infectious Diseases; Tropical Medicine; Entomology;
• 出版者：BioMed Central
• ISSN：1756-3305

文摘

Background The existence nematodes of veterinary importance such as Haemonchus contortus resistant to anthelmintic drugs, including the macrocyclic lactones, has become a major concern in animal health. Macrocyclic lactone resistance in H. contortus seems to be multigenic including the active efflux of these drugs by P-glycoproteins, members of the ABC transporter family, present in this parasite. The goals of the present work were to determine the activity of H. contortus P-glycoprotein 9.1 (Hco-PGP-9.1) and its interaction with the avermectins, ivermectin, abamectin, and also the milbemycin, moxidectin. Additionally, the localisation of Hco-PGP-9.1 was sought in adult worms.