Morphological characteristics and gene mapping of a dense panicle (dp2) mutant in rice (Oryza sativa L.)
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- 作者：Yunlong Yang (1)
Chunhai Shi (1)
Yuanfei Zhou (2)
Jianguo Wu (3)
Xiaoli Jin (1)
Jianyao Shou (1) - 关键词：Dense panicle 2 (dp2) ; Morphological characteristics ; Map ; based cloning ; Rice
- 刊名：Genes & Genomics
- 出版年：2014
- 出版时间：June 2014
- 年：2014
- 卷：36
- 期：3
- 页码：313-319
- 全文大小：
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Chunhai Shi (1)
Yuanfei Zhou (2)
Jianguo Wu (3)
Xiaoli Jin (1)
Jianyao Shou (1)
1. Department of Agronomy, Zhejiang University, Hangzhou, China
2. Agricultural Experiment Station, Zhejiang University, Hangzhou, China
3. Department of Horticulture, Zhejiang A&F University, Linan, China - ISSN：2092-9293
文摘
A dense panicle mutant (dp2) derived from the Oryza sativa ssp. japonica cultivar Nipponbare through ethyl methane sulfonate mutagenesis was used in present study. Compared to the wild type, the panicle of dp2 mutant exhibited more branches and denser grains. Further more, the number of spikelets per panicle, number of primary branches and secondary branches of dp2 mutant were significantly increased while the panicle length, and 1,000-grain weight were significantly decreased. The results from the genetic analysis indicated that the dense panicle phenotype was controlled by a single dominance nuclear gene. Polymorphic analysis of SSR and InDel markers demonstrated that the DP2 gene was located at the long arm of chromosome 2, which was further mapped between SSR markers RM341 and RM13356 in a physical region of 398?kb. Within this region, the RCN2 (LOC_Os02g32950) gene which was annotated relating to the development of rice panicle was found. Compared to the wild type, the sequence of RCN2 gene in the dp2 mutant showed that two SNPs replacement had taken place in the promoter region (G–A) and the intron region (A–T), respectively. The dp2 mutant could be a novel mutant of RCN2 gene and this novel mutant might be useful for further studies on this gene.