Microwave-Mediated Extracellular Synthesis of Metallic Silver and Zinc Oxide Nanoparticles Using Macro-Algae (Gracilaria edulis) Extracts and Its Anticancer Activity Against Human PC3 Cell Lines

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• 作者：Ramaramesh Indra Priyadharshini (1)
  Govindaraj Prasannaraj (1) (2)
  Natesan Geetha (3)
  Perumal Venkatachalam (1) (2)
  
• 关键词：Anticancer activity ; Gracilaria edulis ; Nanoparticle synthesis ; Macro ; algae ; Microwave ; PC3 cell line
• 刊名：Applied Biochemistry and Biotechnology
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：174
• 期：8
• 页码：2777-2790
• 全文大小：2,435 KB
• 参考文献：1. Guarino, M., Rubino, B., & Ballabio, G. (2007). The role of epithelial–mesenchymal transition in cancer pathology. / Pathology, 39, 305-18. CrossRef
  2. Kumar, S., & Kurkjian, C. (2009). Advances in the treatment of metastatic colorectal cancer. / American Journal of Therapy, 16, 412-20. CrossRef
  3. American Cancer Society, 2013. Cancer facts and figures 2013. Available from: www.cancer.org. Atlanta: American Cancer Society.
  4. Balunas, M. J., Su, B., Brueggemeier, R. W., & Kinghorn, A. D. (2008). Natural products as aromatase inhibitors. / Anticancer Agents Medicinal Chemistry, 8, 646-82. CrossRef
  5. Joel, E. L., & Bhimba, V. (2010). Isolation and characterization of secondary metabolites from the mangrove plant / Rhizophora mucronata. Asian Pacific Journal of Tropical Medicine, 3, 602-04. CrossRef
  6. Valery, M. D., Tatyana, A. G., & Vladimir, V. P. (2005). Novel antitumor agents: Marine sponge alkaloids, their synthetic analogs and derivatives. / Mini-Reviews in Medicinal Chemistry, 5, 319-36. CrossRef
  7. Edwin, L. C., & David, Y. (2012). Diving for drugs: Tunicate anticancer compounds. / Drug Discovery Today, 17, 636-48. CrossRef
  8. Kim, S. K., Thomas, N. V., & Li, X. (2011). Anticancer compounds from marine macroalgae and their application as medicinal foods. / Advance Food Nutrition Research, 64, 213-24. CrossRef
  9. Singh, M., Kalaivani, R., Manikandan, S., Sangeetha, N., & Kumaraguru, A. K. (2012). Facile green synthesis of variable metallic gold nanoparticle using / Padina gymnospora, a brown marine macroalga. / Applied Nanoscience, 1-.
  10. Singaravelu, G., Arockiamary, J. S., Kumar, V. G., & Govindaraju, K. (2007). A novel extracellular synthesis of monodisperse gold nanoparticles using marine algae, / Sargassum wightii Greville. / Colloids and Surfaces B: Biointerfaces, 57, 97-01. CrossRef
  11. Rajasulochana, P., Dhamotharan, R., Murugakoothan, P., Murugesan, S., & Krishnamoorthy, P. (2010). Biosynthesis and characterization of gold nanoparticles using the alga / Kappaphycus alvarezii. International Journal of Nanoscience, 9, 511-16. CrossRef
  12. Vivek, M., Senthil Kumar, P., Steffi, S., & Sudha, S. (2011). Biogenic silver nanoparticles by / Gelidiella acerosa extract and their antifungal effects. / Avecenna Journal of Medical Biotechnology, 3, 143-48.
  13. Govindaraju, K., Kiruthiga, V., Kumar, V. G., & Singaravelu, G. (2009). Extracellular synthesis of silver nanoparticles by a marine alga, / Sargassum wightii Grevilli and their antibacterial effects. / Journal of Nanoscience and Nanotechnology, 9, 5497-501. CrossRef
  14. Chanda, S., Nair, R., & Chabhadiya, R. (2007). Marine algae: Screening for a potent antibacterial agent. / Journal of Herbal Pharmacotherapy, 7, 73-6.
  15. Madasamy, S., Thangapandi, M., Arunachalam, P., & Grasian, I. (2012). Antiviral effect of fucoidan extracted from the brown seaweed, / Sargassum wightii, on shrimp / Penaeus monodon post larvae against white spot syndrome virus. / Journal of World Aquaculture Society, 43, 697-06. CrossRef
  16. Stella Mary, J., Vinotha, P., & Pradeep, A.M. (2012). Screening for / in vitro cytotoxic activity of seaweed, / Sargassum sp. against Hep-2 and MCF-7 cancer cell lines. / Asian Pacific Journal of Cancer Prevention, 13, 6073-076.
  17. Liu, X., Shao, C., Kong, W., Fang, Y., & Wang, C. (2013). Evaluation of antitumor, immunomodulatory and free radical scavenging effects of a new herbal prescription seaweed complex preparation. / Journal of Ocean Univer
• 作者单位：Ramaramesh Indra Priyadharshini (1)
  Govindaraj Prasannaraj (1) (2)
  Natesan Geetha (3)
  Perumal Venkatachalam (1) (2)
  
  1. Plant Genetic Engineering and Molecular Biology Lab, Department of Biotechnology, Periyar University, Periyar Palkalai Nagar, Salem, 636 011, Tamil Nadu, India
  2. Centre for Nanoscience and Nanotechnology, Periyar University, Salem, 636 011, Tamil Nadu, India
  3. Department of Biotechnology, Mother Teresa Women’s University, Kodaikanal, 624 101, Tamil Nadu, India
  
• ISSN：1559-0291

文摘

A rapid and novel microwave-mediated protocol was established for extracellular synthesis of metallic silver (Ag) and zinc oxide (ZnO) nanoparticles using the extracts of macro-algae Gracilaria edulis (GE) and also examined its anticancer activity against human prostate cancer cell lines (PC3). The formation of silver nanoparticles (GEAgNPs) and zinc oxide nanoparticles (GEZnONPs) in the reaction mixture was determined by ultraviolet-visible spectroscopy. The synthesized Ag and ZnO nanoparticles were characterized by X-ray diffraction, Fourier transform infra-red spectroscopy, energy dispersive X-ray, and field emission scanning electron microscopy. The silver and zinc oxide nanoparticles were spherical and rod-shaped, respectively. Cell viability assays were carried out to determine the cytotoxic effects of AgNPs and ZnONPs against PC3 and normal African monkey kidney (VERO) cell line. The inhibitory concentration values were found to be 39.60, 28.55, 53.99?μg/mL and 68.49, 88.05, 71.98?μg/mL against PC3 cells and Vero cells for AgNPs, ZnONPs, and aqueous G. edulis extracts, respectively, at 48?h incubation period. As evidenced by acridine orange/ethidium bromide staining, the percentage of the apoptotic bodies was found to be 62 and 70?% for AgNPs and ZnONPs, respectively. The present results strongly suggest that the synthesized ZnONPs showed an effective anticancer activity against PC3 cell lines than AgNPs.