Nitrogen fertiliser-induced changes in N₂O emissions are attributed more to ammonia-oxidising bacteria rather than archaea as revealed using 1-octyne and acetylene inhibitors in two arable soils

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• 作者：Qing Wang ; Li-Mei Zhang ; Ju-Pei Shen ; Shuai Du ; Li-Li Han…
• 关键词：Nitrous oxide (N2O) emission ; Nitrification inhibitors ; Quantitative PCR ; amoA gene ; Arable soil
• 刊名：Biology and Fertility of Soils
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：52
• 期：8
• 页码：1163-1171
• 全文大小：2,201 KB
• 刊物类别：Earth and Environmental Science
• 刊物主题：Life Sciences
  Agriculture
  Soil Science and Conservation
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0789
• 卷排序：52

文摘

Nitrification is believed to be one of the major sources of N₂O production emitted from soil. Previous studies showed that both ammonia-oxidising bacteria (AOB) and archaea (AOA) can produce N₂O via nitrification but their relative contributions are still poorly defined. Here, we used acetylene, an inhibitor of AOB and AOA ammonia monooxygenase (AMO), and 1-octyne, a selective inhibitor that specifically inhibits AOB AMO, to investigate how AOB versus AOA contribute to N₂O emissions in two distinct arable soils. Soil amended with ammonium (NH₄+) increased N₂O emissions to a greater extent than nitrate (NO₃−), and acetylene had a greater impact on N₂O emissions in NH₄+-treated soils than that in NO₃−-amended soils, which indicated that nitrification was the dominant N₂O emitting process in these two arable soils. In the alluvial and red soil, the percentage of evolved N₂O after application of NH₄+ by AOB were 70.5 ~ 78.1 % and 18.7 ~ 19.7 % by AOA, respectively. Quantitative PCR revealed that NH₄+ addition stimulated AOB growth, and the growth could be significantly inhibited by acetylene or 1-octyne in the two soils. The stimulation of N₂O emissions by NH₄+ and the relative suppression by inhibitors paralleled fluctuations in the AOB growth. In addition, cumulative N₂O emissions were not correlated with AOA abundance in the two soils. Our results revealed that AOB could contribute more to soil N₂O production than AOA in the NH₄+-amended arable soils.