Global comparative analysis of expressed genes in ovules and leaves of Ginkgo biloba L.

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• 作者：Li Wang ; Zhaogeng Lu ; Weixing Li ; Jing Xu ; Kaige Luo ; Weichao Lu…
• 关键词：Ginkgo biloba ; Leaves ; Ovules ; Transcriptome ; Gene expression
• 刊名：Tree Genetics & Genomes
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：12
• 期：2
• 全文大小：1,333 KB
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• 作者单位：Li Wang (1)
  Zhaogeng Lu (1)
  Weixing Li (1)
  Jing Xu (1)
  Kaige Luo (1)
  Weichao Lu (1)
  Li Zhang (1)
  Biao Jin (1)
  
  1. College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, Jiangsu, 225009, China
  
• 刊物主题：Forestry; Plant Genetics & Genomics; Plant Breeding/Biotechnology; Tree Biology; Biotechnology;
• 出版者：Springer Berlin Heidelberg
• ISSN：1614-2950

文摘

Ovules are an essential reproductive structure in all seed plants. However, limited information is available on the genetics and genomics of ovules in gymnosperms. Here, we used Illumina sequencing to derive comprehensive gene expression profiles for ovules and leaves in the extant basal gymnosperm Ginkgo biloba. A total of 100,124 transcripts, including 77,898 unigenes, were obtained by de novo assembly from leaf cDNA samples. These transcripts were functionally annotated by comparing their sequences to public protein databases as references. For the comparative analysis of gene expression levels between ovules and leaves, digital gene expression tag profiling was used. An analysis of the expressed genes revealed a high proportion of functional genes commonly expressed in both ovules and leaves. Functional gene expression analyses and microscopic observations implied that ovules and leaves share the photosynthetic structures of chloroplasts and stomata, indicating that they are homologous structures. Interestingly, many homologous genes associated with floral development were expressed in ovules, indicating that gymnosperm ovules share some gene regulatory mechanisms with angiosperm floral organs. The genes that showed highly differential expression levels between leaves and ovules were involved in flavonoid biosynthesis, cell division, hormone transport, transcriptional regulation, and starch and sucrose metabolism, and they indicated higher cell growth and division activity in the ovule. Thus, these results provide valuable gene expression information, which will contribute to an enhanced understanding of the diverse biological mechanisms in the ovules and leaves of G. biloba and provide important molecular insights into ovule evolution in early seed plants.